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Series GSE178160 Query DataSets for GSE178160
Status Public on Jan 06, 2022
Title Glucose Starvation Induces a Switch in the Histone Acetylome for Activation of Gluconeogenic and Fat Metabolism GenesĀ [RNA-seq]
Organism Saccharomyces cerevisiae
Experiment type Expression profiling by high throughput sequencing
Summary Acetyl-CoA is a key intermediate in metabolism situated at the intersection of many metabolic pathways. The reliance of histone acetylation on acetyl-CoA enables gene expression to be coordinated with metabolic state. Previous studies have linked abundant histone acetylation to activation of genes involved in cell growth or tumorigenesis. However, under glucose starvation, the extent to which histone acetylation is important for gene expression remains poorly understood. Here, we use a yeast starvation model to unravel a dramatic alteration in global occupancy of histone acetylation following carbon starvation. We observe a shift in the location of histone acetylation marks from growth-promoting genes to genes required for gluconeogenesis and fat metabolism. This switch is mediated by both the histone deacetylase Rpd3 and the Gcn5p/SAGA acetyltransferase. Our findings reveal a striking specificity for histone acetylation in promoting pathways that generate acetyl-CoA for oxidation when intracellular acetyl-CoA is limitingĀ .
 
Overall design RNA-seq to compare between two different conditions and three different strains.
 
Contributor(s) Tu B, Hsieh W
Citation(s) 34995509
Submission date Jun 14, 2021
Last update date Apr 07, 2022
Contact name Wen-Chuan Hsieh
E-mail(s) [email protected]
Organization name UTSouthwestern Medical Center
Department Biochemistry
Lab Tu lab
Street address 5323 Harry Hines Blvd
City Dallas
State/province Texas
ZIP/Postal code 75390
Country USA
 
Platforms (2)
GPL19756 Illumina NextSeq 500 (Saccharomyces cerevisiae)
GPL27812 Illumina NovaSeq 6000 (Saccharomyces cerevisiae)
Samples (16)
GSM5381173 RNA_WT_D1_pe
GSM5381174 RNA_WT_D2_pe
GSM5381175 RNA_WT_S1_pe
This SubSeries is part of SuperSeries:
GSE178161 Glucose Starvation Induces a Switch in the Histone Acetylome for Activation of Gluconeogenic and Fat Metabolism Genes
Relations
BioProject PRJNA737491
SRA SRP324010

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE178160_wt_gcn5_countTable.logCPM.txt.gz 148.4 Kb (ftp)(http) TXT
GSE178160_wt_rpd3_countTable.logCPM.txt.gz 147.0 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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