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| Status |
Public on Jan 17, 2022 |
| Title |
Transcription factor-like 5 is a potential DNA/RNA binding protein essential for maintaining male fertility in mice [ChIP-seq] |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Tissue-specific transcriptional factors often play key roles in the development of specific cell lineages. Transcription factor-like 5 (TCFL5) is a testis-specific protein that contains the basic helix-loop-helix domain but the in vivo functions of TCFL5 remained unknown. Here, we generated CRISPR/Cas9-mediated knockout mice to dissect the function of TCFL5 in mouse testes. mRNA profiles of pachytene spermatocytes from Tcfl5+/+ and Tcfl5+/- mice sere generated by high throughput sequencing. The bound genes of TCFL5 in wild-type mouse testes were analyzed by ChIP-sequencing.
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| Overall design |
Pachytene spermatocytes were isolated from the testis of Tcfl5+/+ and Tcfl5+/- mice respectively. mRNA profiles of these cells were generated by high throughput sequencing, in two duplicate samples, using BGISEQ-500 platform of BGI Tech. Purified DNA by ChIP was also sequenced on the BGISEQ-500 platform of BGI Tech.
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| Contributor(s) |
Xu W, Wu X |
| Citation(s) |
34931239 |
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| Submission date |
Jun 06, 2021 |
| Last update date |
Apr 21, 2022 |
| Contact name |
Weiya Xu |
| E-mail(s) |
[email protected]
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| Organization name |
Nanjing Medical University
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| Department |
State Key Laboratory of Reproductive Medicine
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| Lab |
XinWu
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| Street address |
No. 818, Tianyuan East Road
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| City |
Nanjing |
| State/province |
Jiangsu |
| ZIP/Postal code |
211100 |
| Country |
China |
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| Platforms (1) |
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| Samples (2) |
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| This SubSeries is part of SuperSeries: |
| GSE176240 |
Transcription factor-like 5 is a potential DNA/RNA binding protein essential for maintaining male fertility in mice. |
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| Relations |
| BioProject |
PRJNA735541 |
| SRA |
SRP322931 |
