|
| Status |
Public on Jun 28, 2021 |
| Title |
RNA-seq analysis of mouse pluripotent stem cells derived cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To detect the role of OP9 stromal cells in our optimized 3D self-assembling peptide induction system followed by the OP9 coculture system. First, we used RNA-seq to analyze mouse pluripotent stem cells derived total cells at day5 between the 3D+OP9 and 3D+0.1% group in our hematopoietic differentiation system. In addition, to further evaluate hematopoietic transcriptome differences between Lin-Sca-1+c-kit+CD201+ cells and Lin-Sca-1+c-kit+CD201- cells, we used RNA-seq to analyze mouse pluripotent stem cells derived Lin-Sca-1+c-kit+CD201+ and Lin-Sca-1+c-kit+CD201- cells at day5 in our 3D+OP9 hematopoietic differentiation system.Meanwhile,we used the mouse embryonic stem cell(mESC) as negative control and bone marrow derived Lin-Sca-1+c-kit+CD201+ and fetal liver derived Lin-Sca-1+c-kit+CD201+ as positive control.
|
| |
|
| Overall design |
two groups together: 3 sample of 3D+OP9 and 3 sample of 3D+0.1%gel; five groups together : 3 sample of D5-LSKCD201+ 3 sample of D5-LSKCD201- 3 sample of mESC 3 sample of BM-LSKCD201+ 3 sample of FL-LSKCD201+
|
| |
|
| Contributor(s) |
Shan W, Zeng X |
| Citation(s) |
34147128 |
| |
| Submission date |
May 26, 2021 |
| Last update date |
Jun 28, 2021 |
| Contact name |
Wei Shan |
| E-mail(s) |
[email protected]
|
| Phone |
18768158120
|
| Organization name |
Zhejiang University
|
| Street address |
Bone Marrow Transplantation Center, The First Affiliated Hospital, School of Medicine, Zhejiang University, No.79 Qingchun Road, Hangzhou, Zhejiang, PR China.
|
| City |
Hangzhou |
| State/province |
Zhejiang |
| ZIP/Postal code |
310000 |
| Country |
China |
| |
|
| Platforms (2) |
|
| Samples (21)
|
|
| Relations |
| BioProject |
PRJNA732994 |
| SRA |
SRP321560 |
Less...
More...