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Series GSE174036 Query DataSets for GSE174036
Status Public on Nov 23, 2021
Title Codon-dependent transcriptional changes in response to tryptophan limitation in the tryptophan auxotrophic pathogens Chlamydia trachomatis and Streptococcus pyogenes
Organisms Homo sapiens; Streptococcus pyogenes NZ131
Experiment type Expression profiling by high throughput sequencing
Summary RNAseq analysis was performed for C. trachomatis serovar L2 and S. pyogenes NZ131 in the presence and absence of tryptophan to monitor transcriptional changes.
 
Overall design RNA was collected from C. trachomatis L2 infected cells at 10, 14, and 24h post-infection. At 10hpi, tryptophan or leucine starvation was induced with Indolmycin or AN3365, respectively, or cells were treated with IFN-gamma. Treatment samples were collected at 14 and 24hpi. Comparisons were made over the timecourse series and comparing treatments to untreated at 10hpi. For S. pyogenes NZ131, the bacteria were cultured in the presence or absence of tryptophan for 1hr prior to RNA collection in the presence or absence of rifampicin (Rif) to determine transcript half-life. All data were compared to the amino acid content of the encoded protein for a given gene.
 
Contributor(s) Ouellette SP, Hatch ND, Wood NA, Herrera AL, Chaussee MS
Citation(s) 34904862
Submission date May 07, 2021
Last update date Jan 10, 2022
Contact name Scot P Ouellette
E-mail(s) [email protected]
Organization name University of Nebraska Medical Center
Department Pathology and Microbiology
Street address 985900 Nebraska Medical Center
City Omaha
State/province NE
ZIP/Postal code 68198
Country USA
 
Platforms (3)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
GPL30099 Illumina NextSeq 500 (Streptococcus pyogenes NZ131)
GPL30892 NextSeq 550 (Streptococcus pyogenes NZ131)
Samples (48)
GSM5285059 10h post-infection (hpi) untreated [A1]
GSM5285060 10h post-infection (hpi) untreated [B1]
GSM5285061 10h post-infection (hpi) untreated [C1]
Relations
BioProject PRJNA728014
SRA SRP318873

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Supplementary file Size Download File type/resource
GSE174036_Processed_RNAseq_data_for_Ctr.xlsx 1.9 Mb (ftp)(http) XLSX
GSE174036_Processed_RNAseq_data_for_GAS.xlsx 1.4 Mb (ftp)(http) XLSX
GSE174036_Processed_RNAseq_data_for_GAS_Rif.xlsx 1.8 Mb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data are available on Series record
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