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| Status |
Public on May 19, 2021 |
| Title |
RNA-Sequencing Analysis of ox-LDL-treated Peritoneal Macrophages Transcriptomes |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Since previous stduies demostrated that oxidized phospholipids function as caspase-11 agonists to induce noncanonical inflammasome activation in immune cells and the levels of oxidized phospholipids derived from ox-LDL are largely elevated in atherosclerotic lesions. Purpose:RNA-Sequencing analysis of ox-LDL-treated peritoneal macrophages transcriptomes.Methods and results:The principal component analysis gene expression profiles of the control and ox-LDL-treated groups were clearly distinct. Among the DEGs that met the cutoff criteria of a -log10(false discovery rate (FDR)) > 2 and |log2(fold change (FC)| > 2. A total of 1,388 downregulated and 855 upregulated genes were identified. GSEA showed that the dominant upregulated pathways in ox-LDL-treated macrophages were associated with the IL-1-mediated signaling pathway, response to cytokine stimulus, and granulocyte migrationwe discovered that caspase-11-mediated inflammation signaling was significantly activated in ox-LDL-treated peritoneal macrophages.Conclusions：we verified caspase11associated inflammatory signaling was significantly activated in ox-LDL-treated macrophages.
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| Overall design |
WT mice peritoneal macrophages were isolated on the third day after thioglycollate infusion and then primed with LPS (100ng/ml) followed by 16 hours of ox-LDL(100ug/ml) stimulation .Total RNA was extracted from 5 biological replicates for each group.
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| Contributor(s) |
Jiang M, Sun X, Liu S, Wang Y, Cai J |
| Citation(s) |
33981234 |
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| Submission date |
Apr 02, 2021 |
| Last update date |
May 19, 2021 |
| Contact name |
Mengqing jiang |
| E-mail(s) |
[email protected]
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| Phone |
15364072601
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| Organization name |
The Third Xiangya Hospital of Central South University
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| Department |
cardiology
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| Street address |
tongzipo road 138
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| City |
长沙 |
| ZIP/Postal code |
410000 |
| Country |
China |
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| Platforms (1) |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA719411 |
| SRA |
SRP313275 |
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