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| Status |
Public on Mar 24, 2022 |
| Title |
A translation control module coordinates germline stem cell differentiation with ribosome biogenesis during Drosophila oogenesis [mRNA_RIP-seq] |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Ribosomal defects perturb stem cell differentiation, causing diseases called ribosomopathies. How ribosome levels control stem cell differentiation is not fully known. Here, we discovered three RNA helicases are required for ribosome biogenesis and for Drosophila oogenesis. Loss of these helicases, which we named Aramis, Athos and Porthos, lead to aberrant stabilization of p53, cell cycle arrest and stalled GSC differentiation. Unexpectedly, Aramis is required for efficient translation of a cohort of mRNAs containing a 5’-Terminal-Oligo-Pyrimidine (TOP)-motif, including mRNAs that encode ribosomal proteins and a conserved p53 inhibitor, Novel Nucleolar protein 1 (Non1). The TOP-motif co-regulates the translation of growth-related mRNAs in mammals. As in mammals, the La-related protein co-regulates the translation of TOP-motif containing RNAs during Drosophila oogenesis. Thus, a previously unappreciated TOP-motif in Drosophila responds to reduced ribosome biogenesis to co-regulate the translation of ribosomal proteins and a p53 repressor, thus coupling ribosome biogenesis to GSC differentiation.
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| Overall design |
mRNA-seq of triplicate samples of ovaries expressing FLAG-tagged Larp subjected to RNA immunoprecipitation using anti-FLAG antibody or IgG as a negative control, with 10% of lysate reserved for input.
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| Contributor(s) |
Martin E, Blatt P, Ngyuen E, Lahr R, Selvam S, Yoon HA, Pocchiari T, Emtenani S, Siekhaus DE, Berman A, Fuchs G, Rangan P |
| Citation(s) |
35579517 |
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| Submission date |
Apr 01, 2021 |
| Last update date |
Jun 23, 2022 |
| Contact name |
Prashanth Rangan |
| E-mail(s) |
[email protected]
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| Phone |
5184423485
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| Organization name |
RNA Institute
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| Department |
Biological Sciences
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| Lab |
Rangan Lab
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| Street address |
1400 Washington
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| City |
Albany |
| State/province |
NY |
| ZIP/Postal code |
12222 |
| Country |
USA |
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| Platforms (1) |
| GPL19132 |
Illumina NextSeq 500 (Drosophila melanogaster) |
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| Samples (9)
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| This SubSeries is part of SuperSeries: |
| GSE171350 |
A translation control module coordinates germline stem cell differentiation with ribosome biogenesis during Drosophila oogenesis |
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| Relations |
| BioProject |
PRJNA719157 |
| SRA |
SRP313144 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE171348_RAW.tar |
560.0 Kb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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