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| Status |
Public on Nov 11, 2021 |
| Title |
Topoisomerase 1 activity during mitotic transcription favors the transition from mitosis to G1 [SLAM-seq] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
Other
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| Summary |
Using SLAM-seq to capture de novo synthesized polyadenylated RNAs, we quantitated changes in nascent RNA expression, as well as in total RNA expression.
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| Overall design |
SLAM-seq in asynchronous cells and mitotic cells (two different timepoints: mid and late mitosis, 0 and 40 respectively). To enrich mitotic cells, they were blocked in S phase by addition of 2.5mM thymidine (Sigma, T9250) for 19-24h and, after washing with fresh medium, transferred in fresh medium containing 400ng/ml nocodazole (Sigma, M1404) and incubated for ~14h. Mitotic cells were collected and allowed to progress to mid mitotis (0) and late mitosis (40). All experiments were performed in HCT116 and derived cell lines.
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| Contributor(s) |
Wiegard A, Kuzin V, Cameron DP, Grosser J, Ceribelli M, Mehmood R, Ballarino R, Valant F, Grochowski R, Karabogdan I, Crosetto N, Lindqvist A, Bizard AH, Kouzine F, Natsume T, Baranello L |
| Citation(s) |
34767771 |
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| Submission date |
Mar 30, 2021 |
| Last update date |
Nov 22, 2021 |
| Contact name |
Vladislav Kuzin |
| E-mail(s) |
[email protected]
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| Phone |
+46707428667
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| Organization name |
Karolinska Institute
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| Department |
Cell and Molecular Biology
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| Lab |
Laura Baranello
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| Street address |
Solnavägen 9
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| City |
Solna |
| State/province |
Stockholm |
| ZIP/Postal code |
171 65 |
| Country |
Sweden |
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| Platforms (1) |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE171134 |
Topoisomerase 1 activity during mitotic transcription favors the transition from mitosis to G1 |
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| Relations |
| BioProject |
PRJNA718531 |
| SRA |
SRP312725 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE171133_Counts_DE_async.csv.gz |
413.4 Kb |
(ftp)(http) |
CSV |
| GSE171133_TC_DE_async.csv.gz |
288.2 Kb |
(ftp)(http) |
CSV |
| GSE171133_TC_DE_mitotic-non0.csv.gz |
724.5 Kb |
(ftp)(http) |
CSV |
| GSE171133_counts_DE_mitotic-non0.csv.gz |
965.0 Kb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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