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| Status |
Public on May 26, 2022 |
| Title |
Ectopic histone modification in extra-embryonic tissues influence implantation and placenta development of IVF embryos (RNA-Seq 1) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Assisted reproductive technology (ART), especially in vitro fertilization (IVF) and embryo transfer have been widely applied in the treatment of human infertility. However, the accumulating evidences indicate that IVF is associated with low pregnancy rate, placental defect and the metabolic diseases in offspring. Here, we identify ectopic histone H3K4me3 in extraembryonic ectoderm (ExE) as an important reason for embryo implantation failure and extra-embryonic development abnormalities of IVF embryos. IVF manipulation notably disrupt extra-embryonic tissue-specific gene expression, 334 epiblast (Epi)-specific genes and 24 Epi-specific transcription factors were abnormally expressed in ExE of IVF embryos at embryonic day 7.5 (E7.5). Combined histone modification analysis reveal that ectopic H3K4me3 modification at the Epi-active promoter resulted in increased expression of these genes in ExE of IVF embryos at E7.5. By konckdown the expression of H3K4me3 recruited regulator Kmt2e, which highly expressed in IVF embryos, can improve the development of IVF embryo and reduce the abnormal gene expression in ExE. Therefore, our research identifies the abnormal H3K4me3 modification occupied in extra-embryonic tissue may be an important reason for implantation failure and abnormal placental development of IVF embryo.
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| Overall design |
We performed RNA-seq transcriptomic analysis of NM and IVF embryos at the stage of 2-cell, morula, E3.5 blastocyst (trophectoderm, TE; inner cell mass, ICM), E7.5 embryos (Epiblast, Epi; Extraembryonic ectoderm, ExE)
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| Contributor(s) |
Bai D, Sun J, Liu W, Jiang C, Gao S |
| Citation(s) |
35508139 |
| |
| Submission date |
Mar 04, 2021 |
| Last update date |
May 26, 2022 |
| Contact name |
Jin Sun |
| E-mail(s) |
[email protected]
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| Organization name |
Tongji University
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| Department |
life science and technology
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| Lab |
Jiang
|
| Street address |
siping1239
|
| City |
shanghai |
| ZIP/Postal code |
200092 |
| Country |
China |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (36)
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| This SubSeries is part of SuperSeries: |
| GSE168274 |
Ectopic histone modification in extra-embryonic tissues influence implantation and placenta development of IVF embryos |
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| Relations |
| BioProject |
PRJNA706715 |
| SRA |
SRP309337 |
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