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| Status |
Public on Jul 28, 2021 |
| Title |
RORα is critical for mTORC1 activity in T cell-mediated colitis [ChIP-seq] |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Inflammatory bowel disease (IBD) is multi-factorial chronic intestinal inflammation driven by pathogenic T cells. The mechanisms underlying colitis pathogenicity and anti-TNF therapy resistance are not fully understood. Here we demonstrate that RORα is highly expressed in active UC patients, particularly in those non-responsive to anti-TNF treatment. Rorα deficient CD4+ T cells could not induce severe gut inflammation in a T cell transfer colitis model. Mechanistically, RORα regulated T cell infiltration in colon by promoting T cell migration and inhibiting T cell apoptosis. Meanwhile, genome-wide occupancy and transcriptome analysis revealed that RORα promoted mTORC1 activation. mTORC1 signaling, also hyperactivated in active UC patients, was necessary for T cell-mediated colitis.
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| Overall design |
We constructed RORα-HA-ires-RFP expressing plasmid, and performed ChIP-seq using transfected in vitro cultured Th17 cells.
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| Contributor(s) |
Chi X, Jin W, Bai X, Zhao X, Shao J, Li J, Sun Q, Su B, Wang X, O Yang X, Dong C |
| Citation missing |
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| Submission date |
Mar 04, 2021 |
| Last update date |
Jul 29, 2021 |
| Contact name |
Chen Dong |
| Organization name |
Tsinghua University
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| Department |
Institute for Immunology and School of Medicine
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| Lab |
Dongchen's lab
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| Street address |
Haidian District
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| City |
Beijing |
| ZIP/Postal code |
100084 |
| Country |
China |
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| Platforms (1) |
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| Samples (2) |
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| This SubSeries is part of SuperSeries: |
| GSE168219 |
RORα is critical for mTORC1 activity in T cell-mediated colitis. |
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| Relations |
| BioProject |
PRJNA706572 |
| SRA |
SRP309250 |
