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| Status |
Public on Jun 21, 2021 |
| Title |
RNA degradation is required for the germ cell-to-maternal transition in Drosophila |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
Other
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| Summary |
In sexually reproducing animals, the oocyte contributes a large supply of RNAs that are essential to launch development upon fertilization. The mechanisms that regulate the composition of the maternal RNA contribution during oogenesis are unclear. Here, we show that a subset of RNAs expressed during the early stages of oogenesis is subjected to regulated degradation during oocyte specification. Failure to remove these RNAs results in oocyte dysfunction and death. We identify the RNA-degrading Super Killer complex and No-Go Decay factor Pelota as key regulators of oogenesis via targeted degradation of specific RNAs expressed in undifferentiated germ cells. These regulators target RNAs enriched for cytidine sequences that are bound by the polypyrimidine tract binding protein Half pint. Thus, RNA degradation helps orchestrate a germ cell-to-maternal transition by sculpting RNAs that become part of the maternal contribution to the zygote.
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| |
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| Overall design |
RNA-seq of mRNA and polysome associated mRNA for various mutant conditions
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| |
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| Contributor(s) |
Blatt PC, Wong-Deyrup S, McCarthy A, Breznak S, Hurton MD, Upadhyay M, Bennink B, Camacho J, Lee MT, Rangan P |
| Citation(s) |
33989522 |
| |
| Submission date |
Feb 05, 2021 |
| Last update date |
Aug 03, 2021 |
| Contact name |
Patrick Blatt |
| E-mail(s) |
[email protected]
|
| Organization name |
SUNY Albany
|
| Street address |
1400 Washington Ave
|
| City |
Albany |
| ZIP/Postal code |
12222 |
| Country |
USA |
| |
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| Platforms (2) |
| GPL19132 |
Illumina NextSeq 500 (Drosophila melanogaster) |
| GPL21306 |
Illumina HiSeq 4000 (Drosophila melanogaster) |
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| Samples (31)
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| Relations |
| BioProject |
PRJNA699903 |
| SRA |
SRP304923 |
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