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| Status |
Public on Feb 06, 2021 |
| Title |
NAP1L2 Drives Mesenchymal Stem Cell Senescence and Suppresses Osteogenic Differentiation [RNA-Seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Changes in these genes are probably a consequence of aging, and the real regulators governing BMSC senescence and osteogenesis are still unclear.In the current study, we report that nucleosome assembly-related protein NAP1L2 serves as an important regulator of both the senescence and osteogenic differentiation of BMSCs through inhibition of NF-κB signaling and recruitment of SIRT1 to deacetylate the H3K14ac level on promoters of osteogenic genes. Thus, targeting NAP1L2 using an aging antagonist such as NMN would benefit aging-related disease.
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| Overall design |
Non-target control and knockdown Nap1l2 C3H10T1/2 cells were divided into the following four groups depending on whether osteogenesis induction for 7days is performed: NC, KD-Nap1l2, NC-7day, KD-Nap1l2-7day.And cells were harvested for gene expression profiling. Two replicates were performed.
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| Contributor(s) |
Hu M |
| Citation(s) |
35032339 |
| |
| Submission date |
Feb 05, 2021 |
| Last update date |
Mar 04, 2022 |
| Contact name |
Liu Zhiqiang |
| E-mail(s) |
[email protected]
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| Organization name |
Tianjin Medical University
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| Street address |
22 Qxiangtai Rd
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| City |
Tianjin |
| ZIP/Postal code |
300070 |
| Country |
China |
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| Platforms (1) |
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| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE166244 |
NAP1L2 Drives Mesenchymal Stem Cell Senescence and Suppresses Osteogenic Differentiation |
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| Relations |
| BioProject |
PRJNA699765 |
| SRA |
SRP304879 |
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