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| Status |
Public on Jun 09, 2009 |
| Title |
Transcriptional and posttranscriptional regulation of transcription factor expression in Arabidopsis roots. |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
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| Summary |
Understanding how the expression of transcription factor (TF) genes is modulated is essential for reconstructing gene regulatory networks. There is increasing evidence that sequences other than upstream noncoding can contribute to modulating gene expression, but how frequently they do so remains unclear. Here, we investigated the regulation of TFs expressed in a tissue-enriched manner in Arabidopsis roots. For 61 TFs, we created GFP reporter constructs driven by each TF's upstream noncoding sequence (including the 5'UTR) fused to the GFP reporter gene alone or together with the TF's coding sequence. We compared the visually detectable GFP patterns with endogenous mRNA expression patterns, as defined by a genome-wide microarray root expression map.
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| Overall design |
To obtain a comprehensive non-overlapping root expression map which would be compared with promoter GFP fusion lines by imaging, we identified three cell type specific GFP marker lines and profiled transcripts in selected cell types using a cell sorting-microarray technique.
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| Contributor(s) |
Lee J, Wang J, Benfey P |
| Citation(s) |
16581911 |
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| Submission date |
Jun 05, 2009 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Ji-Young Lee |
| E-mail(s) |
[email protected]
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| Phone |
607-254-5453
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| Organization name |
Boyce Thompson Institute
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| Street address |
Tower Rd.
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| City |
Ithaca |
| State/province |
NY |
| ZIP/Postal code |
14853 |
| Country |
USA |
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| Platforms (1) |
| GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
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| Samples (8)
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| GSM413907 |
phloem sieve cells and companion cells, biological rep1 |
| GSM413908 |
phloem sieve cells and companion cells, biological rep2 |
| GSM413909 |
cortex, biological rep1 |
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| Relations |
| BioProject |
PRJNA116051 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE16468_RAW.tar |
27.8 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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