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| Status |
Public on Jan 06, 2021 |
| Title |
Transcriptome analysis of KM-H2 cells with different Rhodamin 123 eflux capacity |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Rhodamin 123 is a dye which can be used to detect the activity of ABC transporters. We observed that after staining of KM-H2 Hodgkin lymphoma cells with Rhodamin 123, part of the cells rapidly eliminated the dye, while another part of the cells retained the dye for a longer time.
We compared the transcriptome of KM-H2 Hodgkin lymphoma cells with high Rhodamin 123 efflux capacity and KM-H2 cells with low Rhodamin 123 efflux capacity.
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| Overall design |
KM-H2 cells were stained with Rhodamine 123, washed, and incubated for 4 hrs. Thereafter, cells with high Rhodamine 123 efflux capacity and low efflux capacity were separated by flow cytometry. Samples were analyzed using the Clariom D Human platform. Array data was processed by using Transcriptome Analysis Console.
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| Contributor(s) |
Kreinest T, Volkmer I, Staege MS |
| Citation(s) |
33396195 |
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| Submission date |
Jan 05, 2021 |
| Last update date |
Apr 07, 2021 |
| Contact name |
Martin Sebastian Staege |
| E-mail(s) |
[email protected]
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| Phone |
+49-345-5577280
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| Organization name |
Martin-Luther-University Halle-Wittenberg
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| Department |
Universitätsklinik u. Poliklinik für Kinder- u. Jugendmedizin
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| Lab |
Children's Cancer Reserach Center
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| Street address |
Ernst-Grube-Str. 40
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| City |
Halle (Saale) |
| State/province |
Sachsen-Anhalt |
| ZIP/Postal code |
06120 |
| Country |
Germany |
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| Platforms (1) |
| GPL23126 |
[Clariom_D_Human] Affymetrix Human Clariom D Assay [transcript (gene) version] |
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| Samples (2) |
| GSM5006287 |
KM-H2 cells with high Rhodamine 123 efflux capacity |
| GSM5006288 |
KM-H2 cells with low Rhodamine 123 efflux capacity |
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| Relations |
| BioProject |
PRJNA689846 |
