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| Status |
Public on Dec 30, 2020 |
| Title |
Drosophila Hox genes induce melanized pseudo-tumors when misexpressed in hemocytes |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Hox genes are early determinants of cell identity along the anterior-posterior body axis across bilaterians. Several late non-homeotic functions of Hox genes have emerged in a variety of processes involved in organogenesis in several organisms, including mammals. Several studies have reported the misexpression of Hox genes in a variety of malignancies including acute myeloid leukemia. The Hox genes Dfd, Ubx, abd-A and Abd-B were overexpressed via the UAS-Gal4 system using Cg-Gal4, Lsp2-Gal4, He-Gal4 and HmlD3-Gal4 as specific drivers. Genetic interaction was tested by bringing overexpression lines in heterozygous mutant backgrounds of Polycomb and trithorax group factors. Larvae were visually scored for melanized bodies. Circulating hemocytes were quantified and tested for differentiation. Pupal lethality was assessed. Expression of Dfd, Ubx and abd-A, but not Abd-B in the hematopoietic compartment of Drosophila led to the appearance of circulating melanized bodies, an increase in cell number, cell-autonomous proliferation, and differentiation of hemocytes. Pupal lethality and melanized pseudo-tumors were suppressed in Psc1 and esc2 backgrounds while polycomb group member mutations Pc1 and Su(z)123 and trithorax group member mutation TrlR85 enhanced the phenotype. Dfd, Ubx and abd-A are leukemogenic. Mutations in Polycomb and trithorax group members modulate the leukemogenic phenotype.
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| Overall design |
RNA was isolated from blood tissue of Cg-Gal4>CS and Cg-Gal4>UAS-abd-A larvae and total RNA profiles were generated using Illumina NextSeq 500 to obtain paired end libraries of read length 75 X 2 with at least 25 million reads per sample. There were two replicates from blood tissue of Cg-Gal4>CS and three replicates of Cg-Gal4>UAS-abd-A larvae. The sequence reads were quality checked using FastQC, mapped to reference genome (dm6) using STAR, raw counts were calculated using HTSeq and differential expression was performed using DESeq2.
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| Contributor(s) |
Ponrathnam T, Saini R, Mishra RK |
| Citation missing |
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| Submission date |
Dec 29, 2020 |
| Last update date |
Dec 30, 2020 |
| Contact name |
Divya Tej Sowpati |
| E-mail(s) |
[email protected]
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| Organization name |
CSIR Centre for Cellular and Molecular biology
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| Street address |
Habsiguda
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| City |
Hyderabad |
| State/province |
Telangana |
| ZIP/Postal code |
500007 |
| Country |
India |
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| Platforms (1) |
| GPL19132 |
Illumina NextSeq 500 (Drosophila melanogaster) |
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| Samples (5)
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| Relations |
| BioProject |
PRJNA688480 |
| SRA |
SRP299664 |
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