|
| Status |
Public on Jun 01, 2022 |
| Title |
Next generation sequencing of human cholangiocarcinoma cells and associated stem-like component |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The goal of this study is to provide a global transcriptome profiling (RNA-seq) of stem-like subset in human cholangiocarcinoma (CCA). Functional enrichment of CCA stem-like subset was performed by 3D sphere culture (SPH) in CCA cell lines. Comparison to parental CCA cells grown as 2D monolayer is provided.
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| |
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| Overall design |
mRNA profile of 3D cholangio-spheres (SPH) compared to parental 2D monolayer (MON) cells of 4 different cholangiocarcinoma cell lines
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| |
|
| Contributor(s) |
Ramazzotti M, Marra F, Raggi C, Peano C, Correnti M |
| Citation(s) |
33484774 |
| |
| Submission date |
Dec 08, 2020 |
| Last update date |
Jun 01, 2022 |
| Contact name |
Matteo Ramazzotti |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Florence
|
| Department |
Experimental and Clinical Biomedical Sciences
|
| Street address |
viale Morgagni 50
|
| City |
Firenze |
| ZIP/Postal code |
50134 |
| Country |
Italy |
| |
|
| Platforms (2) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
| GPL21697 |
NextSeq 550 (Homo sapiens) |
|
| Samples (26)
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| Relations |
| BioProject |
PRJNA683582 |
| SRA |
SRP297050 |
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