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Status |
Public on Oct 20, 2021 |
Title |
Simple oligonucleotide-based multiplexing of single-cell chromatin accessibility |
Organisms |
Homo sapiens; Mus musculus |
Experiment type |
Genome binding/occupancy profiling by high throughput sequencing Expression profiling by high throughput sequencing
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Summary |
we reported the development of an accurate, easy-to-use and scalable system (SNuBar) for multiplexing single cell ATAC or multi-omics (ATAC&RNA) assays, and applied this method to multiplex therapy combinations in a lung cancer mouse model and macro-spatial regions of normal breast tissues and samples.
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Overall design |
SNuBar multiplexes scATAC-seq and scATAC&RNA-seq with unmodified oligonucleotides, identfies treatment-induced chromatin dynamics in 28 mice with lung tumors, profiles epigenetic heterogeneity of 32 spatial regions in normal breast tissues, identifies TFs that regulated breast cell type identities.
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Contributor(s) |
Wang K, Xiao Z, Yan Y, Navin N |
Citation(s) |
34686316 |
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Submission date |
Dec 07, 2020 |
Last update date |
May 10, 2024 |
Contact name |
Anil K Sood |
Organization name |
MDACC
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Department |
genetics
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Lab |
Navin Lab
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Street address |
6767 Bertner Ave
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City |
Houston |
State/province |
TX |
ZIP/Postal code |
77030 |
Country |
USA |
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Platforms (6)
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GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
GPL16512 |
Illumina HiSeq 2000 (Homo sapiens; Mus musculus) |
GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
GPL19415 |
Illumina NextSeq 500 (Homo sapiens; Mus musculus) |
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Samples (15)
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Relations |
BioProject |
PRJNA683040 |
SRA |
SRP296812 |