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Series GSE16222 Query DataSets for GSE16222
Status Public on May 30, 2009
Title Effects of heat, anoxia, and combined heat-anoxia treatments
Organism Arabidopsis thaliana
Experiment type Expression profiling by array
Summary Anoxia induces several heat shock proteins and a heat pre-treatment can acclimatize Arabidopsis seedlings to a subsequent anoxic treatment. In this work we analyzed the response of Arabidopsis seedlings to anoxia, heat and a combined heat+anoxia stress. A significant overlapping between the anoxic and heat shock responses has been observed by whole-genome microarray analysis.
 
Overall design We treated Arabidopsis seedling, 4-days old, dark germinated with:
-Control (23°C, dark, liquid Murashige-Skoog medium containing 30mM sucrose).
-Heat-treated (38°C for 90 minutes, dark, liquid Murashige-Skoog medium containing 30mM sucrose).
-Anoxia-treated (23°C, under anoxia for 6h, dark, liquid Murashige-Skoog medium containing 30mM sucrose).
-combined heat+Anoxia-treatment (23°C, treated at 38°C for 90 min and thereafter under anoxia for 6h, dark, liquid Murashige-Skoog medium containing 30mM sucrose).

Two biological replicates for each condition.
 
Contributor(s) Perata P, Banti V, Maffessoni F, Loreti E, Alpi A
Citation(s) 20089772
Submission date May 26, 2009
Last update date Aug 28, 2018
Contact name Pierdomenico Perata
E-mail(s) [email protected]
Phone +39050881901
Organization name Sant'Anna School of Advanced Studies
Department Institute of Life Sciences
Lab PlantLab
Street address Via Guidiccioni 8-10
City San Giuliano Terme
State/province Pisa
ZIP/Postal code 56010
Country Italy
 
Platforms (1)
GPL198 [ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array
Samples (8)
GSM407339 Control replicate No. 1
GSM407340 Control replicate No. 2
GSM407341 Heat-treated replicate No. 1
Relations
BioProject PRJNA117165

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE16222_RAW.tar 27.1 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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