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| Status |
Public on Sep 14, 2021 |
| Title |
CD4+ T cells contribute to neurodgeneration in Lewy Body dementia |
| Organism |
Homo sapiens |
| Experiment type |
Other
Expression profiling by high throughput sequencing
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| Summary |
Recent studies indicate a role of the adaptive immune system in Lewy body dementia (LBD). However, the mechanisms regulating T cell brain homing in LBD remain unknown. Here, we demonstrate interaction of T cells with Lewy bodies and with dopaminergic neurons in post-mortem LBD brains. Single cell RNA sequencing of cerebrospinal fluid (CSF) cells identified upregulated expression of C-X-C Motif Chemokine Receptor 4 (CXCR4) in CD4+ T cells in LBD. CSF protein levels of the CXCR4 ligand, C-X-C Motif Chemokine Ligand 12 (CXCL12) were associated with neuroaxonal damage in LBD. Finally, we demonstrate clonal expansion and upregulated Interleukin 17A by CD4+ T cells stimulated with phosphorylated α-synuclein. Cumulatively, these results indicate CXCR4-CXCL12 signaling as a mechanistic target for inhibiting pathological T cell trafficking in LBD.
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| |
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| Overall design |
Examination of transcriptional profile of cells from the cerbrospinal fluid of healthy and diseased patients
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| |
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| Contributor(s) |
David G, Emma T |
| Citation(s) |
34648304 |
| |
| Submission date |
Nov 10, 2020 |
| Last update date |
Feb 15, 2024 |
| Contact name |
Emma Tapp |
| E-mail(s) |
[email protected], [email protected]
|
| Organization name |
Stanford University
|
| Department |
Neurology
|
| Lab |
Wyss-Coray
|
| Street address |
290 Jane Stanford Way
|
| City |
Stanford |
| State/province |
California (CA) |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
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| Platforms (1) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA675845 |
| SRA |
SRP292005 |
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