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| Status |
Public on May 14, 2009 |
| Title |
Accelerated Postnatal Growth Increases Lipogenic Gene Expression and Adipocyte Size in Low-Birth Weight Mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
OBJECTIVE: To characterize the hormonal milieu and adipose gene expression in response to catch-up growth (CUG), a growth pattern associated with obesity and diabetes risk, in a mouse model of low birth weight (LBW). RESEARCH DESIGN AND METHODS: ICR mice were food restricted by 50% from gestational days 12.5-18.5, reducing offspring birth weight by 25%. During the suckling period, dams were either fed ad libitum, permitting CUG in offspring, or food restricted, preventing CUG. Offspring were killed at age 3 weeks, and gonadal fat was removed for RNA extraction, array analysis, RT-PCR, and evaluation of cell size and number. Serum insulin, thyroxine (T4), corticosterone, and adipokines were measured.
RESULTS: At age 3 weeks, LBW mice with CUG (designated U-C) had body weight comparable with controls (designated C-C); weight was reduced by 49% in LBW mice without CUG (designated U-U). Adiposity was altered by postnatal nutrition, with gonadal fat increased by 50% in U-C and decreased by 58% in U-U mice (P < 0.05 vs. C-C mice). Adipose expression of the lipogenic genes Fasn, AccI, Lpin1, and Srebf1 was significantly increased in U-C compared with both C-C and U-U mice (P < 0.05). Mitochondrial DNA copy number was reduced by >50% in U-C versus U-U mice (P = 0.014). Although cell numbers did not differ, mean adipocyte diameter was increased in U-C and reduced in U-U mice (P < 0.01). CONCLUSIONS: CUG results in increased adipose tissue lipogenic gene expression and adipocyte diameter but not increased cellularity, suggesting that catch-up fat is primarily associated with lipogenesis rather than adipogenesis in this murine model.
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| Overall design |
Epididymal fat samples were obtained at age 3 weeks from 5 control mice (CC), 5 mice exposed to in utero undernutrition (UC), and 4 mice exposed to undernutrition in utero and during suckling (UU).
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| Contributor(s) |
Isganaitis E, Jimenez-Chillaron J, Woo M, Chow A, DeCoste J, Vokes M, Liu M, Kasif S, Zavacki A, Leshan RL, Myers MG, Patti M |
| Citation(s) |
19208909 |
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| Submission date |
May 13, 2009 |
| Last update date |
May 04, 2018 |
| Contact name |
Mary Elizabeth Patti |
| E-mail(s) |
[email protected]
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| Phone |
6177351966
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| Fax |
6177322593
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| Organization name |
Joslin Diabetes Center
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| Department |
Research Division
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| Street address |
1 Joslin Place
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
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| Platforms (1) |
| GPL8321 |
[Mouse430A_2] Affymetrix Mouse Genome 430A 2.0 Array |
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| Samples (14)
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| GSM402545 |
Adipose control rep4 |
| GSM402546 |
Adipose control rep5 |
| GSM402547 |
Adipose utero undernutrition rep1 |
| GSM402548 |
Adipose utero undernutrition rep2 |
| GSM402549 |
Adipose utero undernutrition rep3 |
| GSM402550 |
Adipose utero undernutrition rep4 |
| GSM402551 |
Adipose utero undernutrition rep5 |
| GSM402552 |
Adipose undernutrition in utero and during suckling rep1 |
| GSM402553 |
Adipose undernutrition in utero and during suckling rep2 |
| GSM402554 |
Adipose undernutrition in utero and during suckling rep3 |
| GSM402555 |
Adipose undernutrition in utero and during suckling rep4 |
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| Relations |
| BioProject |
PRJNA115511 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE16083_RAW.tar |
25.5 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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