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| Status |
Public on Dec 11, 2020 |
| Title |
I-KCKT allows dissection-free RNA profiling of adult Drosophila intestinal progenitor cells II |
| Organism |
Drosophila melanogaster |
| Experiment type |
Other
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| Summary |
The adult Drosophila intestinal epithelium is a model system for stem cell biology, but its utility is limited by current biochemical methods that lack cell type resolution. Here, we describe a new proximity-based profiling method that relies upon a GAL4 driver, termed intestinal-kickout-GAL4 (I-KCKT-GAL4), exclusively expressed in intestinal progenitor cells. This method used UV cross-linked whole animal frozen powder as its starting material to immunoprecipitate the RNA cargoes of transgenic epitope-tagged RNA binding proteins driven by I-KCKT-GAL4. When applied to the general mRNA-binder, poly(A)-binding protein, the RNA profile obtained by this method identified 98.8% of transcripts found after progenitor cell sorting, and had low background noise despite being derived from whole animal lysate.
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| Overall design |
Examination of the intestinal progenitor transcriptome using PABP CLIP.
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| Contributor(s) |
Kasun B, Jingjing X, Ishara Surangi A, Nicholas Samuel S |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Oct 26, 2020 |
| Last update date |
Dec 11, 2020 |
| Contact name |
Nicholas S Sokol |
| E-mail(s) |
[email protected]
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| Organization name |
Indiana University
|
| Department |
Biology
|
| Lab |
Sokol Lab
|
| Street address |
1001 E 3rd St JH327
|
| City |
Bloomington |
| State/province |
IN |
| ZIP/Postal code |
47405 |
| Country |
USA |
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| Platforms (1) |
| GPL19132 |
Illumina NextSeq 500 (Drosophila melanogaster) |
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| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE160128 |
I-KCKT allows dissection-free RNA profiling of adult Drosophila intestinal progenitor cells |
|
| Relations |
| BioProject |
PRJNA672035 |
| SRA |
SRP288601 |
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