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| Status |
Public on May 06, 2009 |
| Title |
Expression profile analysis of inflammatory response regulated by hepatocyte nuclear factor 4α |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
To obtain a genomic view of hepatocyte nuclear factor-4α (HNF-4α) in the regulation of the inflammatory response, microarray analysis was used to probe the expression profile of an inflammatory response induced by cytokines in a model of knock-down HNF-4α HepG2 cells. The results indicate an extensive role for HNF-4α plays in the regulation of a large number of the liver-specific genes. Majority of genes (71%) affected by cytokine treatment are also affected by HNF-4α knock-down. This significant overlap suggests that HNF-4α may play a role in regulating the cytokine-induced inflammatory response.
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| Overall design |
The different treated HepG2 cells were grouped into 4 groups (four replicates in each group): Group1, Control; Group2, HNF-4α shRNA treated cells; Group3, cytokine treated group; Group4, HNF-4α shRNA and cytokine treatments. RNA extraction and hybridization on Affymetrix microarrays were processed. The expression profiles between different groups were analyzed and compared. In order to explore the function of HNF-4α in the inflammatory response, a set of 170 genes annotated as inflammatory response was obtained from GO (geneotology.org), the enrichments of these inflammatory genes were analyzed in different treated groups.
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| Contributor(s) |
Wang Z, Bishop EP, Burke PA |
| Citation(s) |
21352552 |
| |
| Submission date |
May 06, 2009 |
| Last update date |
Dec 06, 2018 |
| Contact name |
Peter A Burke |
| E-mail(s) |
[email protected]
|
| Phone |
617-414-8056
|
| Fax |
617-414-7398
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| Organization name |
Boston University School of Medicine
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| Department |
Surgery
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| Lab |
Trauma
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| Street address |
850 Harrison Avenue, Dowling 2 South
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02118 |
| Country |
USA |
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| Platforms (1) |
| GPL571 |
[HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA115349 |
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