|
Status |
Public on Oct 22, 2020 |
Title |
Comprehensive analysis of metabolic and mitochondrial characteristics of extraembryonic endoderm cells |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
|
Summary |
The purpose of the study was to identify transcriptomic changes between feeder-free ES and XEN cells. ES cells were cultured feeder-free on 0.1% gelatin in 2iLIF in N2B27 media. XEN cells were also cultured feeder-free on 0.1% gelaton in RPMI1640 supplemented with 15% FBS, 2mM GlutaMAX and BME.
|
|
|
Overall design |
Bulk RNAseq of feeder-free ES and XEN cells under normal conditions
|
|
|
Contributor(s) |
Gatie MI, Kelly GM |
Citation missing |
Has this study been published? Please login to update or notify GEO. |
|
Submission date |
Oct 22, 2020 |
Last update date |
Oct 26, 2020 |
Contact name |
Mohamed I Gatie |
E-mail(s) |
[email protected]
|
Phone |
2262358553
|
Organization name |
University of Western Ontario
|
Department |
Biology
|
Street address |
1151 Richmond street, Chemistry Building Room 003, Dock 11
|
City |
London |
State/province |
Ontario |
ZIP/Postal code |
N6A5B7 |
Country |
Canada |
|
|
Platforms (1) |
|
Samples (6)
|
|
Relations |
BioProject |
PRJNA670688 |
SRA |
SRP288341 |