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| Status |
Public on Nov 24, 2020 |
| Title |
De novo DNA methyltransferase activity in colorectal cancer is directed towards H3K36me3 marked CpG island (H3K36me3 ChIP-Rx_seq) |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
The aberrant gain of DNA methylation at CpG islands (CGIs) is frequently observed in colorectal tumours and may silence the expression of tumour suppressors such as MLH1. Current models propose that these CGIs are targeted by de novo DNA methyltransferases (DNMTs) in a sequence-specific manner but this has not been tested. Using ectopically integrated CGIs, we find that aberrantly methylated CGIs are subject to low levels of de novo DNMT activity in colorectal cancer cells. By delineating DNMT targets, we find that instead de novo DNMT activity is targeted primarily to CGIs marked by the histone modification H3K36me3, a mark associated with transcriptional elongation. These H3K36me3 marked CGIs are heavily methylated in colorectal tumours and the normal colon suggesting that de novo DNMT activity at CGIs in colorectal cancer is focused on similar targets to normal tissues and not greatly remodelled by tumourigenesis.
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| Overall design |
This series contains H3K36me3 ChIP-Rx_seq from human cancer cell lines
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| |
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| Contributor(s) |
Sproul D |
| Citation(s) |
33514701 |
| |
| Submission date |
Sep 23, 2020 |
| Last update date |
Feb 01, 2021 |
| Contact name |
Duncan Sproul |
| Organization name |
University of Edinburgh
|
| Department |
MRC Human Genetics Unit
|
| Street address |
Crewe Road South
|
| City |
Edinburgh |
| State/province |
Mid Lothian |
| ZIP/Postal code |
EH4 2XU |
| Country |
United Kingdom |
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| Platforms (1) |
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| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE158406 |
De novo DNA methyltransferase activity in colorectal cancer is directed towards H3K36me3 marked CpG island |
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| Relations |
| BioProject |
PRJNA665179 |
| SRA |
SRP285086 |
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