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| Status |
Public on May 05, 2021 |
| Title |
LoopSeq Synthetic Long Read Sequencing Uncovers Isoform Modulation in the progression of Colon Cancer [RNA-seq] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
In this study, we used a barcoding-based synthetic long read (SLR) isoform sequencing approach (LoopSeq) to generate sequencing reads sufficiently long and accurate to identify isoforms using standard short read Illumina sequencers.
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| |
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| Overall design |
Amplicon RNA-sequencing targeting on gene FAM104A and PABPC1 across 6 colon cancer samples. ID 1, 2 and 3 are for patient ID. T and M are for tumor and metastasis samples. GRM and PHU are for two enzymes.
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| |
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| Contributor(s) |
Liu S, Wu I, Yu Y, Balamotis M, Ren B, Yehezkel TB, Luo J |
| Citation(s) |
33907296 |
| |
| Submission date |
Jul 29, 2020 |
| Last update date |
May 05, 2021 |
| Contact name |
Shuchang Liu |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Pittsburgh
|
| Street address |
203 Lothrop Street
|
| City |
Pittsburgh |
| State/province |
PA |
| ZIP/Postal code |
15261 |
| Country |
USA |
| |
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| Platforms (1) |
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| Samples (16)
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| This SubSeries is part of SuperSeries: |
| GSE155921 |
LoopSeq Synthetic Long Read Sequencing Uncovers Isoform Modulation in the progression of Colon Cancer |
|
| Relations |
| BioProject |
PRJNA656188 |
| SRA |
SRP276916 |
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