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| Status |
Public on Jul 01, 2021 |
| Title |
Single Cell analysis of the two Human Hematopoietic Stem Cell Populations |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Single cell transcriptomic profiling (sc RNA-seq) of the two Human Hematopoietic Stem Cell Populations: CD34+CD38-CD45RA-CD90+CD49f+ (hereafter CD90+CD49f+) and CD34+CD38-CD45RA-CD90-CD49f+ (hereafter CD90-CD49f+)
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| Overall design |
Purpose: to compare the single cells transcriptomic profiles of the two Human Hematopoietic Stem Cell Populations: CD90+CD49f+ and CD90-CD49f+ cells in order to find common marker genes.
Methods: single cells (from human cord-blood CD34+ cells) were flow-sorted directly into 6x96-well plates (wells A6-H7) of the two CD90+CD49f+ and CD90-CD49f+ cells; 3 independent sorts (S1, S2, S3); 3 plates per cell type.
Multiplexed Nextera XT was used to generate libraries. Each single cell was barcoded then all libraries were pooled per plated for sequencing.
Conclusions: this study led to the discovery of a group of cells expressing a common cell surface antigen.
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| Contributor(s) |
Anjos-Afonso F, Buettner F, Bonnet D |
| Citation(s) |
35440586 |
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| Submission date |
Jul 22, 2020 |
| Last update date |
Jan 26, 2024 |
| Contact name |
Florian Buettner |
| Organization name |
DKFZ
|
| Department |
DKTK Frankfurt
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| Street address |
Theodor-Stern-Kai 7
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| City |
Frankfurt |
| State/province |
Germany |
| ZIP/Postal code |
60590 |
| Country |
Germany |
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| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
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| Samples (516)
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| Relations |
| BioProject |
PRJNA647898 |
| SRA |
SRP273149 |
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