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| Status |
Public on Jul 23, 2020 |
| Title |
Evaluation of the role of Fbxw7 in interferon-gamma (Ifng) signaling in tumor cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
RNA-sequencing followed by gene set enrichment analysis was used to identify pathways enriched or depleted in Fbxw7-deficient cells. We found that virus sensing, as well as type I and II IFN signaling pathways, were among the most significantly downregulated gene sets in Fbxw7-deficient cells compared with controls. These results establish Fbxw7 as a regulator of the dsRNA sensing and IFN signaling pathways.
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| Overall design |
Fbxw7-deficient and control melanoma cells were treated with Ifng (10ng/mL) for 24h. Total RNA was extracted and submitted to the Molecular Biology Core Facility at Dana-Farber Cancer Institute for sequencing.
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| Contributor(s) |
Haq R, Gstalder C |
| Citation(s) |
32371478 |
| |
| Submission date |
Jul 22, 2020 |
| Last update date |
Oct 22, 2020 |
| Contact name |
Rizwan Haq |
| E-mail(s) |
[email protected]
|
| Organization name |
Dana-Farber Cancer Institute
|
| Department |
Medical Oncology
|
| Street address |
450 Brookline Avenue
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA647835 |
| SRA |
SRP273044 |
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