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| Status |
Public on Jul 13, 2020 |
| Title |
Nuclear Role for Human Argonaute-1 as an Estrogen-dependent Transcription Coactivator |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
In mammals, argonaute (AGO) proteins have been characterized for their roles in small RNA-mediated post-transcriptional and also in transcriptional gene silencing. We report here a different role for AGO1 in estradiol-triggered transcriptional activation in human cells. We show that in MCF-7 mammary gland cells AGO1 associates to transcriptional enhancers of estrogen receptor alpha (ERα) and that this association is upregulated by treating the cells with estrogen (E2), displaying a positive correlation with the activation of these enhancers.
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| |
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| Overall design |
Examination of AGO1 binding to chromatin in vehicle or estradiol treated MCF7 cells
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| Contributor(s) |
Gómez Acuña LI, Nazer E, Rodríguez-Seguí SA, Pozzi B, Buggiano V, Marasco LE, Agirre E, He C, Alló M, Kornblihtt AR |
| Citation(s) |
32673398 |
| |
| Submission date |
Jul 12, 2020 |
| Last update date |
Oct 12, 2020 |
| Contact name |
Alberto R Kornblihtt |
| E-mail(s) |
[email protected]
|
| Organization name |
CONICET_Universidad de Buenos Aires
|
| Street address |
Pabellón IFIBYNE, Ciudad Universitaria
|
| City |
Ciudad Autónoma de Buenos Aires |
| ZIP/Postal code |
1428 |
| Country |
Argentina |
| |
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| Platforms (1) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA645722 |
| SRA |
SRP271494 |
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