Expression profiling by high throughput sequencing
Summary
We performed Smart-seq2 scRNAseq on 156 pancreatic tuft cells . We also profiled FACS sorted EpCAM+;CD45+ immune cells and EpCAM-;CD45- non-immune stroma cells from from 3 caerulein-treated KC and 2 caerulein-treated KPouC pancreata by using the 10X Genomics 3' scRNAseq v3.1 kit.
Overall design
single cell transcriptional profiling of pancreatic tuft cells using Smart-seq2 platform, and immume + stromal cells from caerulein-treated KC and KPouC mice pancreas.