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| Status |
Public on Jul 17, 2020 |
| Title |
Screening for the key miRNAs of exosomes that promote tumor progression and metastasis |
| Platform organism |
synthetic construct |
| Sample organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
Intercellular communication is crucial for breast cancer progression and metastasis. Yet, the role of cancer-derived exosomes and their specific miRNAs cargo in mediating intercellular communications needed to be further elucidated.
MiRNAs control gene expression post-transcriptionally and are selectively packaged into cancer-derived exosomes. Therefore, we hypothesized that miRNAs in cancer-derived exosomes were most likely the essential components of intercellular communication. To identify the key miRNAs, we chose metastatic MDA-MB-231 and non-metastatic MCF-7 for comparison. The miRNAs profiling of exosomes derived from these two kinds of cells as well as the endogenous miRNAs profiling of the cells were conducted by microarrays.
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| Overall design |
MCF7 cells, MDA-MB-231 cells, exosomes isolated from the conditioned medium of MCF7 cells, and exosomes isolated from the conditioned medium of MDA-MB-231 cells were subjected to microarray analysis.
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| Contributor(s) |
Shen S, Song Y |
| Citation(s) |
33618729 |
| |
| Submission date |
Jul 08, 2020 |
| Last update date |
Mar 02, 2021 |
| Contact name |
Songjie Shen |
| Organization name |
Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China
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| Department |
Department of Breast Surgery
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| Street address |
No.1 Shuaifuyuan, Doncheng District
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| City |
Beijing |
| State/province |
Beijing |
| ZIP/Postal code |
100730 |
| Country |
China |
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| Platforms (1) |
| GPL21572 |
[miRNA-4] Affymetrix Multispecies miRNA-4 Array [ProbeSet ID version] |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA644938 |
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