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| Status |
Public on Aug 20, 2020 |
| Title |
TRIBE editing reveals specific mRNA targets of eIF4E-BP in Drosophila and in mammals |
| Organisms |
Drosophila melanogaster; Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
4E-BP (eIF4E-BP) represses translation initiation by binding to the 5’cap-binding protein eIF4E and inhibiting its activity. Although 4E-BP has been shown to be important in growth control, stress response, cancer, neuronal activity and mammalian circadian rhythms, it is not understood how it preferentially represses a subset of mRNAs. We successfully used hyperTRIBE (Targets of RNA-binding proteins identified by editing) to identify in vivo 4E-BP mRNA targets in both Drosophila and mammals under conditions known to activate 4E-BP. The protein associates with specific mRNAs, and ribosome profiling data show that mTOR inhibition changes the translational efficiency of 4E-BP TRIBE targets compared to non-targets. In both systems, these targets have specific motifs and are enriched in translation-related pathways, which correlate well with the known activity of 4E-BP and suggest that it modulates the binding specificity of eIF4E and contributes to mTOR translational specificity.
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| Overall design |
We used hyperTRIBE (Targets of RNA-binding proteins identified by editing) to identify in vivo 4E-BP mRNA targets in both Drosophila S2 cells and human prostate cancer cell PC3 under conditions known to activate 4E-BP. We also carried out ribosome profiling after mTOR inhibition.
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| Contributor(s) |
Jin H, Na D, Song W, Rosbash M |
| Citation(s) |
32851185 |
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https://doi.org/10.1126/sciadv.abb8771
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| |
| Submission date |
Jun 26, 2020 |
| Last update date |
Aug 31, 2020 |
| Contact name |
Hua Jin |
| E-mail(s) |
[email protected]
|
| Phone |
86-18500905853
|
| Organization name |
Beijing Institute of Technology
|
| Street address |
Rm 225-1, Bldg Qiushilou, Beijing Institute of Technology,
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| City |
Beijing |
| State/province |
Choose a State or Province |
| ZIP/Postal code |
100081 |
| Country |
China |
| |
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| Platforms (2) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
| GPL19132 |
Illumina NextSeq 500 (Drosophila melanogaster) |
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| Samples (35)
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| Relations |
| BioProject |
PRJNA642123 |
| SRA |
SRP269009 |
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