|
| Status |
Public on Oct 01, 2020 |
| Title |
Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and Mettl5-/- brain Transcriptomes |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to compare NGS-derived brain transcriptome. Methods: Brain mRNA profiles of 28-day-old wild-type (WT) and methyltransferase like 5 knockout (Mettl5−/−) mice were generated by using oligo(dT)-attached magnetic beads enrichemnt. The sequence reads that passed twice quality filters were analyzed at the transcript isoform level with Bowtie2 for quantitative gene analysis and with DEGseq for differentially expressed genes analysis. Results: 6 samples were tested by using the BGISEq-500 platform, and each sample produced 21.45 M data on average. The average ratio of samples to genomes (mm10) was over 96%; A total of 18,491 genes were detected.
|
| |
|
| Overall design |
Brain mRNA profiles of 28-day old wild type (WT) and Mettl5-/- mice
|
| |
|
| Contributor(s) |
Lin S |
| Citation(s) |
35005123 |
| |
| Submission date |
Jun 24, 2020 |
| Last update date |
Jan 12, 2022 |
| Contact name |
shuibin lin |
| E-mail(s) |
[email protected]
|
| Organization name |
The First Affiliated Hospital of Sun Yat-sen University
|
| Department |
Center for Translational Medicine
|
| Street address |
Nonglin street
|
| City |
Guangzhou |
| State/province |
Guangdong |
| ZIP/Postal code |
510080 |
| Country |
China |
| |
|
| Platforms (1) |
|
| Samples (6)
|
|
| This SubSeries is part of SuperSeries: |
| GSE153140 |
Mettl5 mediated 18S rRNA N6-methyladenosine (m6A) modification controls neural development and the learning and memory function |
|
| Relations |
| BioProject |
PRJNA641587 |
| SRA |
SRP268612 |
Less...
More...