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| Status |
Public on Mar 02, 2021 |
| Title |
Antigen-driven EGR2 expression is required for exhausted CD8+ T cell stability and maintenance [ATACseq] |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
In this study, we examined the role of the transcriptional regulator EGR2 in CD8+ T cell exhaustion during chronic viral infection. Flow cytometric and scRNAseq analysis indicated that EGR2 deficient CD8+ T cells had an abnormal effector-like phenotype. To examine whether this was due to epigenetic alterations, we conducted ATACseq analysis on sorted virus-specific tetramer+ CD8+ T cells isolated at day 20 post-infection with chronic LCMV-Cl13 from either littermate control or EGR2 T cell conditional knock-out mice. The resulting data demonstrated that there were substantial epigenetic changes within EGR2 KO CD8+ T cells, suggesting that EGR2 stabilises the exhausted epigenetic state.
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| Overall design |
The open chromatin regions of exhausted wild-type or EGR2 knockout CD8+ T cells were examined by ATACseq.
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| Contributor(s) |
Parish I, Peters TJ |
| Citation(s) |
33986293 |
| |
| Submission date |
Jun 24, 2020 |
| Last update date |
May 20, 2021 |
| Contact name |
Ian Parish |
| Organization name |
Peter MacCallum Cancer Centre
|
| Street address |
305 Grattan Street
|
| City |
Melbourne |
| State/province |
VIC |
| ZIP/Postal code |
3000 |
| Country |
Australia |
| |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE134710 |
Antigen-driven EGR2 expression is required for exhausted CD8+ T cell stability and maintenance |
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| Relations |
| BioProject |
PRJNA641585 |
| SRA |
SRP268610 |
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