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| Status |
Public on Dec 19, 2020 |
| Title |
Single cell RNA sequencing analysis of freshly resected non-small cell lung carcinoma (NSCLC) tissues |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Lung cancer is the leading cause of cancer-related deaths world-wide. ~85% of lung carcinomas are non–small cell lung carcinoma (NSCLC). Tumor cell heterogeneity is very poorly defined. However, it is known to be important for tumor response to cancer therapy and cancer agressivenes. We subjected three NSCLC tumors resected from different patients to Drop-seq in order to 1) elucidate the capability of scRNA-seq analysis in identifying different tumor cell populations; and 2) ascertain the clinical value of the genes which distinguish cancer cells from other cells in the tissue. As anticipated, the tissue composition of independently collected samples varied. Despite deficient populations in some samples, both donor and patient samples contributed to the majority of cell populations. However, cancer cells were all patient-specific. These findings emphasize the utility of single cell gene expression data in identification of tumor cell populations. The collected data might be further used for predicting of drugs specific to the biology of activated pathways and patient outcome.
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| |
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| Overall design |
Examining NSCLC tumor cell populations using single cell RNA-seq
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| |
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| Contributor(s) |
Benevolenskaya EV, Aissa AF, BIslam A |
| Citation(s) |
33712615 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 CA211095 |
Role of KDM5A in pRB-mediated differentiation |
BOARD OF TRUSTEES OF THE UNIVERSITY OF ILLINOIS |
Elizaveta V Benevolenskaya |
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| |
| Submission date |
Apr 10, 2020 |
| Last update date |
Mar 22, 2021 |
| Contact name |
Elizaveta V Benevolenskaya |
| E-mail(s) |
[email protected]
|
| Phone |
312-413-8947
|
| Organization name |
University of Illinois at Chicago
|
| Department |
Biochemistry and Molecular Genetics
|
| Street address |
900 S. Ashland Ave.
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60607 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
|
| Samples (3) |
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| This SubSeries is part of SuperSeries: |
| GSE149383 |
Drug resistant changes at the level of single cells |
|
| Relations |
| BioProject |
PRJNA624325 |
| SRA |
SRP255972 |
