|
| Status |
Public on Dec 19, 2020 |
| Title |
Genome-wide map of H3K4me3 in erlotinib-resistant PC9 cells. |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
The non-small cell lung carcinoma (NSCLC) PC9 cell line is an established preclinical model for tyrosine kinase inhibitors. Recent evidence suggests that the H3K4 demethylase KDM5A protein is required for emerging drug-resistant cell populations. To better understand the dependence of cell survival on H3K4me3 level, we treated the EGFR-mutant PC9 cells with erlotinib and performed ChIP-seq analysis using H3K4me3 antibodies. We were able to identify genomic regions that exhibit decreased H3K4me3 in drug-treated cells. The results of this study will help to address clinical usefulness of KDM5A-targeted strategies in overcoming drug resistance, particularly in NSCLC.
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| |
|
| Overall design |
Genome-wide map of H3K4me3 in erlotinib-resistant PC9 cells.
|
| |
|
| Contributor(s) |
Benevolenskaya EV, Islam AB, Go C |
| Citation(s) |
33712615 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 CA211095 |
Role of KDM5A in pRB-mediated differentiation |
BOARD OF TRUSTEES OF THE UNIVERSITY OF ILLINOIS |
Elizaveta V. Benevolenskaya |
|
| |
| Submission date |
Apr 10, 2020 |
| Last update date |
Mar 22, 2021 |
| Contact name |
Elizaveta V Benevolenskaya |
| E-mail(s) |
[email protected]
|
| Phone |
312-413-8947
|
| Organization name |
University of Illinois at Chicago
|
| Department |
Biochemistry and Molecular Genetics
|
| Street address |
900 S. Ashland Ave.
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60607 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA624314 |
| SRA |
SRP255975 |
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