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| Status |
Public on Feb 23, 2021 |
| Title |
Identification of Fra-1 and/or Fra-2 regulated genes in MDA-MB231 cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
In the paper "Fra-1 regulates its target genes via binding to remote enhancers without exerting major control on chromatin architecture in triple negative breast cancers" by Bejjani et al., we identified Fra-1 and/or Fra-2 target genes in MDA-MB-231 cells. si RNA against Fra-1 and against Fra-2 were transfected in MDA-MB-231 cells either independenlty or simultaneously to identify genes regulated specifically by Fra-1 or Fra-2 and genes regulated redundantly or complementarily by Fra-1 and Fra-2
total RNA were purified and biotinylated sense-strand cDNA were produced. cDNA targets were used to probe Affymetrix GeneChip Human Gene 2.0 ST arrays
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| Overall design |
transcriptome 1 : cells either non transfected (T1.NT) or transfected with control siRNA (T1.siCTL) , siRNA against Fra-1 (T.siFra1) or against Fra-2 (T1.siFra2). 3 independant replicates (A, B and C) for each condition were produced
transcriptome 2 : cells either non transfected (T2.NT) or transfected with control siRNA (T2.siCTL) , with siRNA against Fra-1 + siRNA against Fra-2 (T2.siFra1_siFra2). 3 independant replicates (A, B and C) for each condition were produced
to discard bias linked to siRNA transfection itself, as well as potential bias linked to control siRNA , we introduced another control, i.e. cells that received the transfection reagent but not transfected (NT) with siRNA. Fold changes were calculated versus NT conditions for the different siRNA transfections
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| Web link |
https://doi.org/10.1093/nar/gkab053
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| Contributor(s) |
Bejjani F, Tolza C, Kaoma T, Vallar L, Piechaczyk M, Jariel-Encontre I |
| Citation(s) |
33533919 |
| |
| Submission date |
Mar 11, 2020 |
| Last update date |
Apr 06, 2021 |
| Contact name |
Isabelle Jariel-Encontre |
| E-mail(s) |
[email protected]
|
| Phone |
00 33 (0)4 11 28 31 53
|
| Organization name |
IRCM-U1194- INSERM
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| Street address |
208 Avenue des Apothicaires
|
| City |
Montpellier |
| State/province |
Montpellier Cedex5 |
| ZIP/Postal code |
34298 |
| Country |
France |
| |
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| Platforms (1) |
| GPL16686 |
[HuGene-2_0-st] Affymetrix Human Gene 2.0 ST Array [transcript (gene) version] |
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| Samples (21)
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| This SubSeries is part of SuperSeries: |
| GSE146825 |
Transcriptional regulation by Fra-1 in triple negative breast cancers |
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| Relations |
| BioProject |
PRJNA612053 |
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