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GEO help: Mouse over screen elements for information. |
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| Status |
Public on May 05, 2020 |
| Title |
Cas12a base editors induce efficient and specific editing with low DNA damage response (DNA targeted sequencing) |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Other
|
| Summary |
The advent of base editors (BEs) holds a promising potential in correcting pathogenic-related point mutations to treat relevant diseases. Unexpectedly, Cas9 nickase (nCas9) derived BEs lead to DNA double-strand breaks, which can trigger unwanted cellular responses including a p53-mediated DNA damage response (DDR). Here, we showed that catalytically-dead-Cas12a (dCas12a) conjugated BEs induced no DNA break and minimally activated DDR proteins including H2AX, ATM, ATR and p53. We further developed a BEACON (Base Editing induced by human APOBEC3A and Cas12a without DNA break) system that fuses dCas12a to the engineered APOBEC3A with enhanced deamination efficiency and editing specificity. By using BEACON, efficient C-to-T editing was achieved at levels comparable to AncBE4max and only low levels of DDR and RNA off-target (OT) effects were triggered in mammalian cells. BEACON also induced in vivo base editing in mouse embryos and targeted C-to-T conversions were detected in F0 mice.
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| Overall design |
Examination of indels and base substitutions induced by the Cas12a base editors
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| Contributor(s) |
Yang L, Chen J, Liu Z, Yang B, Xiong Y, Wang X |
| Citation(s) |
32492431 |
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| Submission date |
Feb 19, 2020 |
| Last update date |
Aug 05, 2020 |
| Contact name |
Li Yang |
| E-mail(s) |
[email protected]
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| Organization name |
Fudan University
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| Department |
Institutes of Biological Sciences
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| Street address |
131 Dong-An Road
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| City |
Shanghai |
| ZIP/Postal code |
200032 |
| Country |
China |
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| Platforms (5)
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| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
| GPL19415 |
Illumina NextSeq 500 (Homo sapiens; Mus musculus) |
|
| Samples (248)
|
| GSM4321121 |
rA1-dCas12a-BE:VEG-b, TET, S6, S3, RUN, RAG, PD1, DYR, DNM, CFT, CDK rep a |
| GSM4321122 |
rA1-dCas12a-BE:S4, RAG, FAN rep a; VEG-b, S6, RUN, DNM, CFT, CDK rep b |
| GSM4321123 |
rA1-dCas12a-BE:S4, PD1 rep b; S6, RUN, CFT rep c; VEG-a rep a |
| GSM4321124 |
rA1-dCas12a-BE:PD1, CDK rep c; VEG-a, TET, S3, RAG, RAG, FAN, DYR rep b |
| GSM4321125 |
rA1-dCas12a-BE:VEG-b, VEG-a, TET, S4, S3, RAG, RAG, FAN, DYR, DNM rep c |
| GSM4321126 |
hA3A-dCas12a-BE: VEG-b, TET, S3, RUN, RAG, DYR, DNM, CFT, CDK rep a |
| GSM4321127 |
hA3A-dCas12a-BE:CDK rep e; S4, RAG, PD1, FAN rep a; S6 rep d; VEG-b, RUN, DYR, DNM, CFT rep b |
| GSM4321128 |
hA3A-dCas12a-BE:DNM rep d; RUN, CFT rep c; S4, PD1, CDK rep b; VEG-a, S6 rep a |
| GSM4321129 |
hA3A-dCas12a-BE:PD1, CDK rep c; RUN rep g; S6, DNM rep e; VEG-a, TET, S3, RAG, RAG, FAN rep b |
| GSM4321130 |
hA3A-dCas12a-BE:RUN rep h; S6, CDK rep f; VEG-b, VEG-a, TET, S4, S3, RAG, RAG, FAN, DYR, DNM rep c |
| GSM4321131 |
hA3B-dCas12a-BE:VEG-b, TET, S6, S3, RUN, RAG, DYR, DNM, CFT, CDK rep a |
| GSM4321132 |
hA3B-dCas12a-BE:S4, RAG, PD1, FAN rep a; VEG-b, S6, RUN, DNM, CFT, CDK rep b |
| GSM4321133 |
hA3B-dCas12a-BE:S4, PD1 rep b; S6, RUN, CFT rep c and VEG-a rep a |
| GSM4321134 |
hA3B-dCas12a-BE:PD1, CDK rep c; VEG-a, TET, S3, RAG, RAG, FAN, DYR rep b |
| GSM4321135 |
hA3B-dCas12a-BE:VEG-b, VEG-a, TET, S4, S3, RAG, RAG, FAN, DYR, DNM rep c |
| GSM4321136 |
hAID-dCas12a-BE:VEG-b, TET, S6, S3, RUN, RAG, FAN, DYR, DNM, CFT, CDK rep a |
| GSM4321137 |
hAID-dCas12a-BE:S4, RAG, PD1 rep a; VEG-b, S6, RUN, FAN, DNM, CFT, CDK rep b |
| GSM4321138 |
hAID-dCas12a-BE:S4, PD1 rep b; S6, RUN, CFT rep c; VEG-a rep a |
| GSM4321139 |
hAID-dCas12a-BE:PD1, FAN, CDK rep c; VEG-a, TET, S3, RAG, RAG, DYR rep b |
| GSM4321140 |
hAID-dCas12a-BE:VEG-b, VEG-a, TET, S4, S3, RAG, RAG, DYR, DNM rep c |
| GSM4321141 |
NT:VEG-b, VEG-a, TET, S6, S4, S3, RUN, RAG, RAG, PD1, FAN, DYR, DNM, CFT, CDK rep a |
| GSM4321142 |
NT:VEG-b, VEG-a, TET, S6, S4, S3, RUN, RAG, RAG, PD1, FAN, DYR, DNM, CFT, CDK rep b |
| GSM4321143 |
NT:VEG-b, VEG-a, TET, S6, S4, S3, RUN, RAG, RAG, PD1, FAN, DYR, DNM, CFT rep c |
| GSM4321144 |
NT:CDK rep c |
| GSM4321145 |
hA3A-dCas12a-BE: S4, CDK rep d and S6 rep b |
| GSM4321146 |
hA3A(W98A)-dCas12a-BE:S6, S4, DYR, CDK rep a |
| GSM4321147 |
hA3A(W98Y)-dCas12a-BE:S6, S4, DYR, CDK rep a |
| GSM4321148 |
hA3A(W104A)-dCas12a-BE:S6, DYR, CDK rep a |
| GSM4321149 |
hA3A(W104Y)-dCas12a-BE:S6, S4, DYR, CDK rep a |
| GSM4321150 |
hA3A(P134A)-dCas12a-BE:S6, S4, DYR, CDK rep a |
| GSM4321151 |
hA3A(P134Y)-dCas12a-BE:S6, S4, DYR, CDK rep a |
| GSM4321152 |
hA3A-dCas12a-BE: CDK rep h; DYR rep d; S4 rep e; S6 rep c |
| GSM4321153 |
hA3A(W98A)-dCas12a-BE:S6, S4, DYR, CDK rep b |
| GSM4321154 |
hA3A(W98Y)-dCas12a-BE:S6, S4, DYR, CDK rep b |
| GSM4321155 |
hA3A(W104A)-dCas12a-BE:S4 rep a; S6, CDK rep b; S6, S4 rep d |
| GSM4321156 |
hA3A(W104Y)-dCas12a-BE:S6, S4, DYR, CDK rep b |
| GSM4321157 |
hA3A(P134A)-dCas12a-BE:S6, S4, DYR, CDK rep b |
| GSM4321158 |
hA3A(P134Y)-dCas12a-BE:S6, S4, DYR, CDK rep b |
| GSM4321159 |
hA3A(W104A)-dCas12a-BE: DYR rep b |
| GSM4321160 |
hA3A-dCas12a-BE: CDK rep I; DYR rep e; S4 rep f |
| GSM4321161 |
hA3A(W98A)-dCas12a-BE:S6, S4, CDK rep c |
| GSM4321162 |
hA3A(W98Y)-dCas12a-BE:S6, S4, CDK rep c |
| GSM4321163 |
hA3A(W104A)-dCas12a-BE:S4 rep b; S4 rep e; S6, CDK rep c |
| GSM4321164 |
hA3A(W104Y)-dCas12a-BE:CDK rep c |
| GSM4321165 |
hA3A(P134A)-dCas12a-BE:CDK rep c |
| GSM4321166 |
hA3A(P134Y)-dCas12a-BE:CDK rep c |
| GSM4321167 |
hA3A(W104A)-dCas12a-BE:DYR rep c |
| GSM4321168 |
hA3A-dCas12a-BE:DYR rep f |
| GSM4321169 |
hA3A(W104A)-dCas12a-BE:S4 rep c,f |
| GSM4321170 |
hA3A(W104Y)-dCas12a-BE:S6 rep c |
| GSM4321171 |
hA3A(P134Y)-dCas12a-BE:S6, S4 rep c |
| GSM4321172 |
hA3A(W98A)-dCas12a-BE:DYR rep c |
| GSM4321173 |
hA3A(W98Y)-dCas12a-BE:DYR rep c |
| GSM4321174 |
hA3A(W104Y)-dCas12a-BE:S4, DYR rep c |
| GSM4321175 |
hA3A(P134A)-dCas12a-BE:S6, S4, DYR rep c |
| GSM4321176 |
hA3A(P134Y)-dCas12a-BE:DYR rep c |
| GSM4321177 |
hA3A-dCas12a-BE: EMX rep h; S4, DNM rep g |
| GSM4321178 |
hA3A(W98Y/W104A)-dCas12a-BE:S6, RUN, PD1, CDK rep a |
| GSM4321179 |
hA3A(W98Y/P134Y)-dCas12a-BE:S4 rep a |
| GSM4321180 |
hA3A(W104A/P134Y)-dCas12a-BE:S6, S4, RUN, PD1, EMX, DYR, CDK rep a |
| GSM4321181 |
hA3A(W98Y/W104A/P134Y)-dCas12a-BE:S4, RUN, EMX, DYR, CDK rep a |
| GSM4321182 |
hA3A-dCas12a-BE:PD1, EMX rep i |
| GSM4321183 |
hA3A(W98Y/W104A)-dCas12a-BE:EMX, DYR rep a;RUN, PD1, CDK rep b |
| GSM4321184 |
hA3A(W98Y/P134Y)-dCas12a-BE:S4 rep b and S6, RUN, PD1, DYR rep a |
| GSM4321185 |
hA3A(W104A/P134Y)-dCas12a-BE:DNM rep a; S4, DYR, CDK rep b |
| GSM4321186 |
hA3A(W98Y/W104A/P134Y)-dCas12a-BE:S4, EMX, CDK rep b; S6, PD1, DNM rep a |
| GSM4321187 |
hA3A-dCas12a-BE: RUN rep f; S6, DYR rep i; hA3A(W98Y/W104A)-dCas12a-BE: S6 rep b |
| GSM4321188 |
hA3A(W98Y/W104A)-dCas12a-BE:EMX, DYR rep b; RUN, PD1, CDK rep c; S4, DNM rep a |
| GSM4321189 |
hA3A(W98Y/P134Y)-dCas12a-BE:EMX, DNM, CDK rep a; S4 rep c; S6, RUN, PD1 rep b |
| GSM4321190 |
hA3A(W104A/P134Y)-dCas12a-BE:DYR, CDK rep c; S6, RUN, PD1, EMX, DNM rep b |
| GSM4321191 |
hA3A(W98Y/W104A/P134Y)-dCas12a-BE:PD1, DNM rep b; S4, CDK rep c |
| GSM4321192 |
hA3A-dCas12a-BE: S4 rep h |
| GSM4321193 |
hA3A(W98Y/W104A)-dCas12a-BE:S4, DNM rep b |
| GSM4321194 |
hA3A(W98Y/P134Y)-dCas12a-BE:EMX, DYR, DNM, CDK rep b; PD1 rep c |
| GSM4321195 |
hA3A(W104A/P134Y)-dCas12a-BE: S6, S4, RUN, EMX rep c |
| GSM4321196 |
hA3A(W98Y/W104A/P134Y)-dCas12a-BE:DNM rep c; S6, RUN, DYR rep b |
| GSM4321197 |
hA3A-dCas12a-BE:DYR rep g; S4 rep i |
| GSM4321198 |
hA3A(W98Y/W104A)-dCas12a-BE:S6, S4, EMX, DYR, DNM rep c |
| GSM4321199 |
hA3A(W98Y/P134Y)-dCas12a-BE:S6, RUN, EMX, DYR, DNM, CDK rep c |
| GSM4321200 |
hA3A(W104A/P134Y)-dCas12a-BE:PD1, DNM rep c |
| GSM4321201 |
hA3A(W98Y/W104A/P134Y)-dCas12a-BE:S6, RUN, PD1, EMX, DYR rep c |
| GSM4321202 |
hA3A-dCas12a-BE:DNM rep i; EMX rep d; PD1 rep e |
| GSM4321203 |
hA3A(W98A)-dCas12a-BE:PD1, EMX, DNM rep a |
| GSM4321204 |
hA3A(W98Y)-dCas12a-BE:RUN, PD1, EMX, DNM rep a |
| GSM4321205 |
hA3A(W104A)-dCas12a-BE:DNM rep a |
| GSM4321206 |
hA3A(W104Y)-dCas12a-BE:RUN, PD1 rep a |
| GSM4321207 |
hA3A(P134A)-dCas12a-BE:PD1, EMX, DNM rep a |
| GSM4321208 |
hA3A(P134Y)-dCas12a-BE:RUN, PD1, EMX rep a |
| GSM4321209 |
hA3A-dCas12a-BE:DNM rep f; EMX rep e |
| GSM4321210 |
hA3A(W98A)-dCas12a-BE:PD1, EMX, DNM rep b; RUN rep a |
| GSM4321211 |
hA3A(W98Y)-dCas12a-BE:RUN, EMX, DNM rep b |
| GSM4321212 |
hA3A(W104A)-dCas12a-BE:DNM rep b; RUN rep d; RUN, EMX rep a |
| GSM4321213 |
hA3A(W104Y)-dCas12a-BE:EMX, DNM rep a; RUN, PD1 rep b |
| GSM4321214 |
hA3A(P134A)-dCas12a-BE:PD1 rep b; RUN rep a |
| GSM4321215 |
hA3A(P134Y)-dCas12a-BE:DNM rep a; PD1 rep b |
| GSM4321216 |
hA3A-dCas12a-BE:PD1 rep g; PD1, EMX rep f; RUN rep d, i |
| GSM4321217 |
hA3A(W98A)-dCas12a-BE:RUN rep b |
| GSM4321218 |
hA3A(W98Y)-dCas12a-BE:EMX rep c; PD1 rep b |
| GSM4321219 |
hA3A(W104A)-dCas12a-BE:DNM rep c; PD1 rep a; RUN rep b, e |
| GSM4321220 |
hA3A(W104Y)-dCas12a-BE:EMX, DNM rep b |
| GSM4321221 |
hA3A(P134A)-dCas12a-BE:RUN rep b |
| GSM4321222 |
hA3A(P134Y)-dCas12a-BE:EMX, DNM rep b |
| GSM4321223 |
hA3A-dCas12a-BE:DNM rep h; EMX rep g |
| GSM4321224 |
hA3A(W98A)-dCas12a-BE:PD1, DNM rep c |
| GSM4321225 |
hA3A(W98Y)-dCas12a-BE:RUN rep c |
| GSM4321226 |
hA3A(W104A)-dCas12a-BE:PD1, EMX rep b |
| GSM4321227 |
hA3A(W104Y)-dCas12a-BE:RUN, EMX, DNM rep c |
| GSM4321228 |
hA3A(P134A)-dCas12a-BE:EMX, DNM rep b; RUN rep c |
| GSM4321229 |
hA3A(P134Y)-dCas12a-BE:PD1, EMX rep c; RUN rep b |
| GSM4321230 |
hA3A-dCas12a-BE: PD1 rep h; RUN rep e |
| GSM4321231 |
hA3A(W98A)-dCas12a-BE:RUN, EMX rep c |
| GSM4321232 |
hA3A(W98Y)-dCas12a-BE:PD1, DNM rep c |
| GSM4321233 |
hA3A(W104A)-dCas12a-BE:RUN rep f; RUN, PD1, EMX rep c |
| GSM4321234 |
hA3A(W104Y)-dCas12a-BE:PD1 rep c |
| GSM4321235 |
hA3A(P134A)-dCas12a-BE:PD1, EMX, DNM rep c |
| GSM4321236 |
hA3A(P134Y)-dCas12a-BE:RUN, DNM rep c |
| GSM4321237 |
HSD rA1-dCas12a-BE mouse tail#1 |
| GSM4321238 |
HSD rA1-dCas12a-BE mouse tail#2 |
| GSM4321239 |
HSD rA1-dCas12a-BE mouse tail#3; hA3A(W104A)-dCas12a-BE-op:S6, S4, RUN, DYR, CDK rep a |
| GSM4321240 |
HSD rA1-dCas12a-BE mouse tail#4 |
| GSM4321241 |
HSD rA1-dCas12a-BE mouse tail#5; hA3A(W98Y/W104A)-dCas12a-BE-op:S6, S4, RUN rep a |
| GSM4321242 |
HSD rA1-dCas12a-BE mouse tail#6; S4, hA3A(W104A/P134Y)-dCas12a-BE-op:RUN, DYR, CFT rep a |
| GSM4321243 |
HSD rA1-dCas12a-BE mouse tail#7; BEACON2:S6, DYR, CDK rep a |
| GSM4321244 |
HSD rA1-dCas12a-BE mouse tail#8; hA3A(W98Y/W104A/Y132D)-dCas12a-BE-op:S6, CDK rep a |
| GSM4321245 |
hA3A(W104A/Y130F/P134Y)-dCas12a-BE-op:CDK rep a; HSD BEACON1 mouse tail#1 |
| GSM4321246 |
hA3A(W104A/Y132D/P134Y)-dCas12a-BE-op:DYR, CDK rep a; HSD BEACON1 mouse tail#2 |
| GSM4321247 |
hA3A(W98Y/W104A/Y130F/Y132D)-dCas12a-BE-op:CDK rep a; HSD BEACON1 mouse tail#3 |
| GSM4321248 |
HSD BEACON1 mouse tail#4 |
| GSM4321249 |
HSD BEACON1 mouse tail#5; hA3A-dCas12a-BE:S6 rep g |
| GSM4321250 |
HSD BEACON1 mouse tail#6 |
| GSM4321251 |
hA3A(W104A)-dCas12a-BE-op:CFT rep a;S6, RUN, DYR rep b; HSD BEACON1 mouse tail#7 |
| GSM4321252 |
hA3A(W98Y/W104A)-dCas12a-BE-op:DYR, CFT, CDK rep a;S6, RUN rep b; HSD BEACON1 mouse tail#9; |
| GSM4321253 |
HSD BEACON1 mouse tail#10; hA3A(W104A/P134Y)-dCas12a-BE-op:RUN, DYR rep b;S6, CDK rep a |
| GSM4321254 |
HSD BEACON1 mouse tail#11; BEACON2:S4, RUN, CFT rep a;S6, DYR, CDK rep b |
| GSM4321255 |
HSD BEACON1 mouse tail#12; hA3A(W98Y/W104A/Y132D)-dCas12a-BE-op:S4, RUN, DYR, CFT rep a |
| GSM4321256 |
hA3A(W104A/Y130F/P134Y)-dCas12a-BE-op:CDK rep b;S6, S4, RUN, DYR, CFT; HSD BEACON1 mouse tail#13 |
| GSM4321257 |
hA3A(W104A/Y132D/P134Y)-dCas12a-BE-op:DYR rep b;S6, S4, RUN, CFT rep a; HSD BEACON1 mouse tail#14 |
| GSM4321258 |
hA3A(W98Y/W104A/Y130F/Y132D)-dCas12a-BE-op:CDK rep b; S6, S4, RUN, DYR, CFT rep a; HSD BEACON1 mouse tail#15 |
| GSM4321259 |
HSD BEACON1 mouse tail#16-tail |
| GSM4321260 |
HSD BEACON1 mouse tail#17 |
| GSM4321261 |
HSD BEACON1 mouse tail#18 |
| GSM4321262 |
hA3A(W104A)-dCas12a-BE-op:DYR rep c; S4, CFT, CDK rep b; HSD BEACON1 mouse tail#19 |
| GSM4321263 |
HSD BEACON1 mouse tail#20 |
| GSM4321264 |
HSD BEACON1 mouse tail#21; hA3A(W98Y/W104A)-dCas12a-BE-op:S4, DYR, CFT, CDK rep b;S6, RUN rep c |
| GSM4321265 |
HSD BEACON1 mouse tail#22; hA3A(W104A/P134Y)-dCas12a-BE-op:RUN, DYR rep c; S6, S4, CFT, CDK rep b |
| GSM4321266 |
HSD BEACON1 mouse tail#23; BEACON2:S4, RUN, CFT rep b;S6, DYR, CDK rep c |
| GSM4321267 |
HSD BEACON1 mouse tail#24; hA3A(W98Y/W104A/Y132D)-dCas12a-BE-op:S6, S4, RUN, DYR , CFT, CDK rep b |
| GSM4321268 |
hA3A(W104A/Y130F/P134Y)-dCas12a-BE-op:CDK rep c; S6, S4, RUN, DYR, CFT rep b |
| GSM4321269 |
hA3A(W104A/Y132D/P134Y)-dCas12a-BE-op:S6, S4, RUN, CFT, CDK rep b |
| GSM4321270 |
hA3A(W98Y/W104A/Y130F/Y132D)-dCas12a-BE-op:CDK rep c; S6, S4, RUN, DYR, CFT rep b |
| GSM4321271 |
hA3A-dCas12a-BE:CDK rep g; PD1 rep d; S6, DYR rep h |
| GSM4321272 |
hA3A(W104A)-dCas12a-BE-op: S6, S4, RUN, CFT, CDK rep c |
| GSM4321273 |
HSD rA1-dCas12a-BE mouse embryo#1; hA3A(W98Y/W104A)-dCas12a-BE-op:S4, DYR, CFT, CDK rep c |
| GSM4321274 |
HSD rA1-dCas12a-BE mouse embryo#2; hA3A(W104A/P134Y)-dCas12a-BE-op:S6, S4, CFT, CDK rep c |
| GSM4321275 |
HSD rA1-dCas12a-BE mouse embryo#3; BEACON2:S4, RUN, CFT rep c |
| GSM4321276 |
HSD rA1-dCas12a-BE mouse embryo#4; hA3A(W98Y/W104A/Y132D)-dCas12a-BE-op:S6, S4, RUN, DYR, CFT, CDK rep c |
| GSM4321277 |
HSD rA1-dCas12a-BE mouse embryo#5; hA3A(W104A/Y130F/P134Y)-dCas12a-BE-op:S6, S4, RUN, DYR, CFT rep c |
| GSM4321278 |
HSD rA1-dCas12a-BE mouse embryo#6; hA3A(W104A/Y132D/P134Y)-dCas12a-BE-op: S6, S4, RUN, DYR, CFT, CDK rep c |
| GSM4321279 |
HSD rA1-dCas12a-BE mouse embryo#7; hA3A(W98Y/W104A/Y130F/Y132D)-dCas12a-BE-op: S6, S4, RUN, DYR, CFT rep c |
| GSM4321280 |
HSD rA1-dCas12a-BE mouse embryo#8 |
| GSM4321281 |
HSD BEACON1 mouse heart#7; BE3:PAF, CHD, BMR, BLM rep a |
| GSM4321282 |
HSD BEACON1 mouse liver#7; BE3:BMR, BLM BE3 rep b; SPA, PMS, PDE rep a |
| GSM4321283 |
HSD BEACON1 mouse spleen#7; BE3:BMR, BLM BE3 rep c; SPA, PMS, PDE, PAF, CHD rep b |
| GSM4321284 |
HSD BEACON1 mouse lung#7; BE3:SPA, PMS, PDE, PAF, CHD rep c |
| GSM4321285 |
hA3A(W104A)-dCas12a-BE:CDK rep d;S6 rep e; AncBE4max: SPA, PMS, PDE, PAF, BMR rep a; HSD BEACON1 mouse kindey#7 |
| GSM4321286 |
hA3A(W104A)-dCas12a-BE:CDK rep e;AncBE4max:CHD, BLM rep a;SPA,PMS,PDE,PAF,BMR rep b;DYR rep d;HSD BEACON1 mouse brain#7 |
| GSM4321287 |
hA3A(W104A)-dCas12a-BE:DYR rep e;AncBE4max:CHD, BLM rep b; SPA, PMS, PDE, PAF, BMR rep c; HSD BEACON1 mouse testis#7 |
| GSM4321288 |
HSD rA1-dCas12a-BE mouse embryo#9; hA3A(W104A)-dCas12a-BE:S6, DYR, CDK rep f; AncBE4max:CHD, BLM rep c |
| GSM4321289 |
HSD rA1-dCas12a-BE mouse embryo#10 |
| GSM4321290 |
HSD rA1-dCas12a-BE mouse embryo#11 |
| GSM4321291 |
HSD BEACON1 mouse tail#8 |
| GSM4321292 |
HSD BEACON1 embryo#1;hA3A(W104A/Y130F)-dCas12a-BE-op:S6,S4,RUN,DYR,CDK;rA1-dCas12a-BE:SPA,PMS,PDE,PAF,CHD,BMR,BLM |
| GSM4321293 |
hA3A(W104A/Y130F)-dCas12a-BE-op:CFT,S6,S4,RUN,DYR,CDK; rA1-dCas12a-BE:SPA, PMS, PDE, PAF, CHD, BMR;HSD BEACON1 embryo#2 |
| GSM4321294 |
hA3A(W104A/Y130F)-dCas12a-BE-op:CFT,S6,S4,RUN,DYR; rA1-dCas12a-BE:BLM, SPA, PMS, PDE, PAF, CHD, BMR;HSD BEACON1 embryo#3 |
| GSM4321295 |
hA3A(W104A/Y130F)-dCas12a-BE-op:CFT, CDK rep c; HSD BEACON1 mouse embryo#4;rA1-dCas12a-BE:BLM rep c |
| GSM4321296 |
HSD BEACON1 mouse embryo#5;BEACON1:S6, S4, RUN, DYR, CFT, CDK rep a;BEACON2:PMS, PAF, CHD rep a |
| GSM4321297 |
HSD BEACON1 mouse embryo#6;BEACON1:S6, S4, RUN, DYR, CFT, CDK rep b;BEACON2:PMS, PAF, CHD rep b |
| GSM4321298 |
HSD BEACON1 mouse embryo#7;BEACON1:S6, S4, RUN, DYR, CFT, CDK rep c;BEACON2:PAF, CHD rep c |
| GSM4321299 |
HSD BEACON1 mouse embryo#8;BEACON2:PMS rep c |
| GSM4321300 |
NT: SPA, PMS, PDE, PAF, CHD, BMR, BLM rep a |
| GSM4321301 |
NT: SPA, PMS, PDE, PAF, CHD, BMR, BLM rep b |
| GSM4321302 |
NT: SPA, PMS, PDE, PAF, CHD, BMR, BLM rep c |
| GSM4321303 |
BE2:PMS, PDE, CHD, BMR, BLM rep a; Cas9:VEG rep a |
| GSM4321304 |
YE1-BE3:PDE, CHD, BMR, BLM rep a; BE2:VEG rep a |
| GSM4321305 |
BE3:VEG rep a |
| GSM4321306 |
BEACON1:PMS rep b; SPA, PDE, BMR, BLM rep a |
| GSM4321307 |
BEACON2:SPA, BMR, BLM rep a; rA1-dCas12a-BE: VEG rep a |
| GSM4321308 |
BE2:PDE, CHD, BMR, BLM rep b; SPA, PAF rep a |
| GSM4321309 |
YE1-BE3:PDE, CHD, BMR, BLM rep b;SPA, PMS, PAF rep a |
| GSM4321310 |
BEACON1:PMS, PAF, CHD rep a; SPA, BMR rep b; Cas9:VEG rep b |
| GSM4321311 |
BEACON2:PDE rep a;SPA, BMR rep b; BE2:VEG BE2 rep b; Cas9:VEG rep c |
| GSM4321312 |
BE2: PDE, CHD, BLM rep c; SPA, PMS, PAF rep b; BE3:VEG rep b |
| GSM4321313 |
YE1-BE3:PDE, CHD, BMR, BLM rep c; SPA, PMS, PAF rep b |
| GSM4321314 |
hA3A(C101S)-dCas12a-BE:S6, S4, RUN, PD1, EMX, DYR, DNM, CDK rep a; rA1-dCas12a-BE: VEG rep b |
| GSM4321315 |
hA3A(C101S)-dCas12a-BE:S4, RUN, PD1, EMX, DYR, DNM, CDK rep b;BEACON1: PDE, PAF, CHD, BLM rep b; SPA, BMR rep c |
| GSM4321316 |
hA3A(C101S)-dCas12a-BE:S4, RUN, PD1, EMX, DYR, DNM, CDK rep c;S6 rep b;BEACON2: PDE, BLM rep b; SPA, BMR rep c |
| GSM4321317 |
hA3A(C101S)-dCas12a-BE:S6 rep c; BE2:SPA, PMS, PAF, BMR rep c |
| GSM4321318 |
hA3A(C106S)-dCas12a-BE:S6, S4, RUN, PD1, EMX, DYR, DNM, CDK rep a; YE1-BE3:SPA, PMS, PAF rep c |
| GSM4321319 |
hA3A(C106S)-dCas12a-BE:S6, S4, RUN, EMX, DYR, DNM, CDK rep b;BE2:VEG rep c |
| GSM4321320 |
hA3A(C106S)-dCas12a-BE:PD1repb;S6,S4,RUN,EMX,DYR,DNM,CDKrepc;BEACON1:PMS,PDE,PAF,CHD,BLMrepc;BE3:VEGrepc |
| GSM4321321 |
hA3A(C106S)-dCas12a-BE:PD1 rep c;BEACON2:PDE, BLM rep c |
| GSM4321322 |
rA1-dCas12a-BE:VEG rep c |
| GSM4321323 |
SCN, HSD BEACON2 mouse embryo#1 |
| GSM4321324 |
SCN, HSD BEACON2 mouse embryo#2 |
| GSM4321325 |
SCN, HSD BEACON2 mouse embryo#3 |
| GSM4321326 |
SCN, HSD BEACON2 mouse embryo#4 |
| GSM4321327 |
SCN, HSD BEACON2 mouse embryo#5 |
| GSM4321328 |
SCN, HSD BEACON2 mouse embryo#6 |
| GSM4321329 |
SCN, HSD BEACON2 mouse embryo#7 |
| GSM4321330 |
SCN, HSD BEACON2 mouse embryo#8 |
| GSM4321331 |
SCN, HSD BEACON2 mouse embryo#9 |
| GSM4321332 |
SCN, HSD BEACON2 mouse embryo#10 |
| GSM4321333 |
SCN, HSD BEACON2 mouse embryo#11 |
| GSM4321334 |
SCN, HSD BEACON2 mouse embryo#12 |
| GSM4321335 |
SCN, HSD BEACON2 mouse embryo#13 |
| GSM4321336 |
SCN, HSD BEACON2 mouse embryo#14 |
| GSM4321337 |
SCN BEACON2 mouse embryo#15 |
| GSM4321338 |
SCN BE3 mouse embryo#1 |
| GSM4321339 |
SCN BE3 mouse embryo#2 |
| GSM4321340 |
SCN BE3 mouse embryo#3 |
| GSM4321341 |
SCN BE3 mouse embryo#4 |
| GSM4321342 |
SCN BE3 mouse embryo#5 |
| GSM4321343 |
SCN BE3 mouse embryo#6 |
| GSM4321344 |
SCN BE3 mouse embryo#7 |
| GSM4321345 |
SCN BE3 mouse embryo#8 |
| GSM4321346 |
SCN BE3 mouse embryo#9 |
| GSM4321347 |
SCN BE3 mouse embryo#10 |
| GSM4321348 |
SCN BE3 mouse embryo#11 |
| GSM4321349 |
SCN BE3 mouse embryo#12 |
| GSM4321350 |
SCN BE3 mouse embryo#13 |
| GSM4321351 |
SCN BE3 mouse embryo#14 |
| GSM4321352 |
SCN BE3 mouse embryo#15 |
| GSM4321353 |
SCN BE3 mouse embryo#16 |
| GSM4321354 |
SCN AncBE4max mouse embryo#1 |
| GSM4321355 |
SCN AncBE4max mouse embryo#2 |
| GSM4321356 |
SCN AncBE4max mouse embryo#3 |
| GSM4321357 |
SCN AncBE4max mouse embryo#4 |
| GSM4321358 |
SCN AncBE4max mouse embryo#5 |
| GSM4321359 |
SCN AncBE4max mouse embryo#6 |
| GSM4321360 |
SCN AncBE4max mouse embryo#7 |
| GSM4321361 |
SCN AncBE4max mouse embryo#8 |
| GSM4321362 |
SCN AncBE4max mouse embryo#9 |
| GSM4321363 |
SCN AncBE4max mouse embryo#10 |
| GSM4321364 |
SCN AncBE4max mouse embryo#11 |
| GSM4321365 |
SCN AncBE4max mouse embryo#12 |
| GSM4321366 |
SCN AncBE4max mouse embryo#13 |
| GSM4321367 |
SCN AncBE4max mouse embryo#14 |
| GSM4321368 |
SCN AncBE4max mouse embryo#15 |
|
| This SubSeries is part of SuperSeries: |
| GSE145552 |
Cas12a base editors induce efficient and specific editing with low DNA damage response |
|
| Relations |
| BioProject |
PRJNA607539 |
| SRA |
SRP250019 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE145550_Indels.xls.gz |
89.3 Kb |
(ftp)(http) |
XLS |
| GSE145550_RAW.tar |
44.7 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
| Processed data are available on Series record |
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