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Series GSE14496 Query DataSets for GSE14496
Status Public on Dec 15, 2009
Title A combinatorial Interplay Among the ACC Synthase Isoforms Regulates Ethylene Biosynthesis in Arabidopsis thaliana
Organism Arabidopsis thaliana
Experiment type Expression profiling by array
Summary ACC Synthase (ACS) is the key regulatory enzyme in the ethylene biosynthesis in plants. It catalyzes the conversion of s-adenosylmethionine (SAM) to 1-aminocyclopropane-1-carboxylic acid (ACC), the precursor of ethylene. Arabidopsis has nine ACS genes. The goal of the project is to inactivate each gene by insertional mutagenesis and amiRNA technology and eventually construct a null ACS mutant. We have been recently able to achieve this goal. Furthermore, we wanted to know how inactivation of individual ACS genes affects global gene expression.

Keywords: ACS mutant comprrison; global gene expression.
 
Overall design Triplicate samples of 10-day light grown seedling from each ACS mutant was used for microarray analysis.
 
Contributor(s) Tsuchisaka A, Yu G, Theologis A
Citation(s) 19752216
Submission date Jan 21, 2009
Last update date Aug 15, 2018
Contact name Atsunari Tsuchisaka
E-mail(s) [email protected], [email protected]
Phone 510-559-5924
Organization name University of California, Berkeley / USDA
Department Plant Gene Expression Center
Street address 800 Buchanan St.
City Albany
State/province CA
ZIP/Postal code 94710
Country USA
 
Platforms (1)
GPL198 [ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array
Samples (42)
GSM362024 col control, biological rep1 (single mutant)
GSM362025 col control, biological rep2 (single mutant)
GSM362026 col control, biological rep3 (single mutant)
Relations
Affiliated with GSE69995
BioProject PRJNA111553

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE14496_RAW.tar 91.1 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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