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| Status |
Public on Jan 15, 2020 |
| Title |
Joint Profiling of Chromatin Accessibility and CAR-T Integration Site Analysis at Population and Single-cell Levels I |
| Organism |
Homo sapiens |
| Experiment type |
Other
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
We report the development of a novel method called EpiVIA (https://github.com/VahediLab/epiVIA) for the joint profiling of the chromatin accessibility and lentiviral integration site analysis at the population and single-cell levels. We validate our technique in clonal cells with previously defined integration sites and further demonstrate the ability to measure lentiviral integration sites and chromatin accessibility of host and viral genomes at the single-cell resolution in CAR-T cells. We anticipate that EpiVIA will enable the single-cell deconstruction of gene regulation during CAR-T therapy, leading to the discovery of cellular factors associated with durable treatment.
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| |
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| Overall design |
Idenfification of integration sites in two clonal cells with ATAC-seq.
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| |
|
| Contributor(s) |
Wang W, Vahedi G |
| Citation(s) |
32094195 |
| |
| Submission date |
Jan 14, 2020 |
| Last update date |
Mar 30, 2020 |
| Contact name |
Wenliang Wang |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Pennsylvania
|
| Street address |
421 Curie Boulevard
|
| City |
Philadelphia |
| State/province |
PENNSYLVANIA |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
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| Platforms (1) |
|
| Samples (2) |
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| This SubSeries is part of SuperSeries: |
| GSE143647 |
Joint Profiling of Chromatin Accessibility and CAR-T Integration Site Analysis at Population and Single-cell Levels |
|
| Relations |
| BioProject |
PRJNA601178 |
| SRA |
SRP241923 |
