|
| Status |
Public on Oct 24, 2019 |
| Title |
Single cell RNAseq of adoptively transferred Tregs in rhesus macaques |
| Organism |
Macaca mulatta |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Animal R.401 was infused with ex-vivo expanded T-regulatory cells. After adoptive transfer, PBMC were obtained and stained with surface antibodies. Single cell libraries from sorted populations were generated using a UMI-based droplet-partitioning platform (10X Genomics) and sequenced using a NextSeq 500 (Illumina). Resulting reads were processed using Cellranger software (10X Genomics) and downstream analysis was performed using Monocle6,7 using a negative binomial model of distribution with fixed variance.
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| |
|
| Overall design |
Time-course
|
| |
|
| Contributor(s) |
Furlan S |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| BioProject |
PRJNA577285 |
| |
| Submission date |
Oct 21, 2019 |
| Last update date |
Oct 24, 2019 |
| Contact name |
Geoff R Hill |
| Organization name |
Fred Hutchinson Cancer Research Center
|
| Department |
CRD
|
| Lab |
Hill Lab
|
| Street address |
1100 Fairview Ave. N.
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98109 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL21120 |
Illumina NextSeq 500 (Macaca mulatta) |
|
| Samples (8)
|
|
| Relations |
| SRA |
SRP225421 |
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