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| Status |
Public on Oct 17, 2022 |
| Title |
Makorin 1 controls embryonic patterning by alleviating Bruno1-mediated repression of oskar translation |
| Organism |
Drosophila melanogaster |
| Experiment type |
Other
|
| Summary |
Makorins are evolutionary conserved proteins that contain C3H-type zinc finger modules and a RING E3 ubiquitin ligase domain. In Drosophila maternal Makorin 1 (Mkrn1) has been linked to embryonic patterning but the mechanism remained unsolved. Here, we show that Mkrn1 is essential for axis specification and pole plasm assembly by translational activation of oskar. We demonstrate that Mkrn1 interacts with poly(A) binding protein (pAbp) and binds specifically to osk 3’ UTR in regions overlapping Bruno1 (Bru1) responsive elements (BREs), which regulate osk translation. We observe increased association of the translational repressor Bru1 with osk mRNA upon depletion of Mkrn1, indicating that both proteins compete for osk binding. Consistently, reducing Bru1 dosage partially rescues viability and Osk protein level in ovaries from Mkrn1 females. We conclude that Mkrn1 controls embryonic patterning and germ cell formation by specifically activating osk translation, most likely by competing with Bru1 to bind to osk 3’ UTR.
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| Overall design |
iCLIP analysis of Mkrn1 in ovaries
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| |
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| Contributor(s) |
Dold A, Hänel H, Busch A, Brüggemann M, Zarnack K, König J, Roignant J |
| Citation missing |
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| |
| Submission date |
Oct 17, 2019 |
| Last update date |
Oct 19, 2022 |
| Contact name |
Annabelle Dold |
| Organization name |
Institute of Molecular Biology, IMB
|
| Lab |
Roignant
|
| Street address |
Ackermannweg 4
|
| City |
Mainz |
| ZIP/Postal code |
55128 |
| Country |
Germany |
| |
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| Platforms (1) |
| GPL19132 |
Illumina NextSeq 500 (Drosophila melanogaster) |
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| Samples (5)
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| Relations |
| BioProject |
PRJNA578112 |
| SRA |
SRP226066 |
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