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| Status |
Public on Mar 20, 2009 |
| Title |
Transcriptome changes triggered by the synthetic defense elicitors DCA and INA in Arabidopsis thaliana |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
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| Summary |
DCA (3,5-Dichloroanthranilic acid) is a newly identified synthetic defense elicitor. To perform a comparative analysis of defense responses triggered by DCA and the structurally related defense inducer INA (2,6-Dichloroisonicotinic acid) Affymetrix chip experiments were performed with Arabidopsis thaliana seedlings treated with one of these two compounds.
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| Overall design |
Arabidopsis thaliana plants (accession Col-0) were grown on soil in a semi-sterile growth chamber under fluorescent lights (14 hours light, 10 hours dark, 21 centi grades, 100 Einstein/m2/s). Wild type Col-0 plants and the npr1-3 mutant were used in this study. Aerial tissues of 2 week old soil grown Arabidopsis seedlings were sprayed with 100uM 3,5-Dichloroanthranilic acid (DCA), or 100uM 2,6-Dichloroisonicotinic acid (INA) or mock solution and harvested 48h or 6 days after the treatment. Final DMSO concentrations never exceeded 0.002%. Mock treatments were application of 0.002% DMSO in water. For harvesting the aerial plant parts were shock-frozen in liquid nitrogen. Total RNA was isolated from seedlings using TRIZOL (Invitrogen) follwing the manufacturer’s instructions. RNA was processed and hybridized to Affymetrix Arabidopsis ATH1 genome array GeneChip following manufacturer’s instructions (Affymetrix) by the University of California, Riverside Core Instrument Facility. Three independent biological replicates were performed for each treatment.
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| Contributor(s) |
Knoth C, Girke T, Eulgem T |
| Citation(s) |
19304930 |
| |
| Submission date |
Dec 04, 2008 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Thomas Eulgem |
| E-mail(s) |
[email protected]
|
| Phone |
951 827 7740
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| Organization name |
University of California
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| Department |
Botany and Plant Sciences
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| Lab |
Eulgem Lab
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| Street address |
University of California, Department of Botany and Plant Sciences
|
| City |
Riverside |
| State/province |
CA |
| ZIP/Postal code |
92521 |
| Country |
USA |
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| Platforms (1) |
| GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
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| Samples (24)
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| Relations |
| Affiliated with |
GSE69995 |
| BioProject |
PRJNA110511 |
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