|
| Status |
Public on Apr 22, 2010 |
| Title |
Interaction between the light environment and the Arabidopsis wound response |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
|
| Summary |
We have previously identified a significant increase in chloroplast reactive oxygen species in wounded leaves of Arabidopsis and other plants, which is light-dependent (Flor-Henry et al. (2004) BMC Plant Biology 4:19). The aims of this study were to (i) examine the early response to mechanical wounding in Arabidopsis leaves, (ii) test the hypothesis that light-dependent chloroplast ROS may play a role in signalling for changes in gene expression in wounded leaves, and (iii) examine the broader impact of the light environment on the wound response in Arabidopsis.
Keywords: Stress response
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| |
|
| Overall design |
The experiment was a two-by-two factorial design, with wounding and light as the two variables. In each replicate, there are 4 treatments: unwounded/light, unwounded/dark, wounded/light, wounded/dark. Three independent biological replicate experiments were performed, with one array hybridisation performed per treatment.
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| |
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| Contributor(s) |
Roberts MR, Morker K, Worrall D |
| Citation(s) |
21241328 |
| |
| Submission date |
Dec 03, 2008 |
| Last update date |
Aug 15, 2018 |
| Contact name |
Rekin's Janky |
| E-mail(s) |
[email protected]
|
| Organization name |
VIB
|
| Department |
Nucleomics Core
|
| Street address |
Herestraat 49 Box 816
|
| City |
Leuven |
| ZIP/Postal code |
B-3000 |
| Country |
Belgium |
| |
|
| Platforms (1) |
| GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
|
| Samples (12)
|
| GSM347109 |
Arabidopsis rosette_Light Control_Rep1 |
| GSM347110 |
Arabidopsis rosette_Dark Wounded_Rep1 |
| GSM347111 |
Arabidopsis rosette_Dark Control_Rep1 |
| GSM347112 |
Arabidopsis rosette_Light Wounded_Rep1 |
| GSM347113 |
Arabidopsis rosette_Light Control_Rep2 |
| GSM347114 |
Arabidopsis rosette_Light Wounded_Rep2 |
| GSM347115 |
Arabidopsis rosette_Dark Control_Rep2 |
| GSM347116 |
Arabidopsis rosette_Dark Wounded_Rep2 |
| GSM347117 |
Arabidopsis rosette_Light Control_Rep3 |
| GSM347118 |
Arabidopsis rosette_Light Wounded_Rep3 |
| GSM347119 |
Arabidopsis rosette_Dark Control_Rep3 |
| GSM347120 |
Arabidopsis rosette_Dark Wounded_Rep3 |
|
| Relations |
| Affiliated with |
GSE69995 |
| BioProject |
PRJNA110567 |
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