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| Status |
Public on Mar 01, 2020 |
| Title |
Development of gene expression signatures using herbal drug ninjin'yoeito (NYT) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
To identify hematopoietic genes regulated by NYT, microarray analysis was carried out to compare BM MNCs cultured with and without NYT for 11 days.
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| Overall design |
BM MNCs cultured with NYT or negative controls were collected at day 11. Total RNA was extracted using RNeasy Plus Micro Kit (QIAGEN). RNA concentration was measured by NANODROP 2000c (Thermo Fisher Scientific). cRNA was amplified, labeled, and hybridized to an Agilent Whole Mouse GE 4 9 44K v2 Microarray (Agilent Technologies, Santa Clara, CA, USA) according to the manufacturer’s instructions. All hybridized microarrays were scanned using an Agilent scanner, and signals of all probes were analyzed using Feature Extraction Software (Agilent Technologies).
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| Contributor(s) |
Sugiyama D |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Sep 05, 2019 |
| Last update date |
Mar 03, 2020 |
| Contact name |
Daisuke Sugiyama |
| Organization name |
Kyushu university
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| Department |
Incubation Center for Advanced Medical Science (ICAMS)
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| Street address |
3-1-1, Maidashi, Higasi-ku
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| City |
Fukuoka |
| State/province |
Fukioka |
| ZIP/Postal code |
812-8587 |
| Country |
Japan |
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| Platforms (1) |
| GPL11202 |
Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Probe Name version) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA564049 |
