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| Status |
Public on Jul 07, 2020 |
| Title |
scRNA-seq of T cell Independent B cell responses |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To fine map the continuum of molecular changes that occur as B cells differentiate to antibody secreting plasma cells in response to the T cell independent antigens LPS and NP-Ficoll we performed scRNA-seq. WT or IRF4-deficient B cells were CTV labeled and adoptively transferred into congenically disparate uMT hosts. One day later, the mice were innoculated with LPS and all transferred cells isolated at 72 hours and profiled on the 10x Genomimcs 5' Library Kit. In addition, we adoptively transferred WT B cells into congenically disparate WT hosts and innoculated the animals with either LPS or NP-Ficoll, and 72 hours later performed scRNA-seq on all responding B cells.
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| Overall design |
scRNA-seq on two independent biological replicates of wild-type or IRF4-deficient B cells responding to LPS in uMT mice and wild-type B cells responding to LPS or NP-Ficoll in wild-type hosts.
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| Contributor(s) |
Scharer CD, Boss JM |
| Citation(s) |
32778653 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| P01 AI125180 |
Plasma Cells in Health and Disease |
EMORY UNIVERSITY |
Ignacio E. Sanz |
| R01 AI123733 |
Epigenetic Regulation of Plasma Cells Differentiation |
EMORY UNIVERSITY |
JEREMY M. BOSS |
| F31 AI138391 |
Epigenetic Regulation of Memory B Cell Differentiation and Reactivation |
EMORY UNIVERSITY |
Madeline Joan Price |
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| |
| Submission date |
Aug 26, 2019 |
| Last update date |
Aug 13, 2020 |
| Contact name |
Chris Scharer |
| E-mail(s) |
[email protected]
|
| Organization name |
Emory University
|
| Department |
Microbiology and Immunology
|
| Lab |
Chris Scharer
|
| Street address |
1510 Clifton Rd, Suite 3086A
|
| City |
Atlanta |
| State/province |
GA |
| ZIP/Postal code |
30322 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA562315 |
| SRA |
SRP219696 |
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