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| Status |
Public on Oct 10, 2019 |
| Title |
RNA-seq of human foreskin fibroblast cells lacking RB, p130, and p107 treated with doxorubicin. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We used human foreskin fibroblast cells with CRISPR-Cas9 mediated knockout of RB1 and p130 (RBL2) (sgP130, sgRB1+sg130), knocked down p107 using siRNA and measured gene expression changes after doxorubicin treatmnet (24 hr, 350 nM).
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| Overall design |
sgP130 and sgRB1+sgP130 cells were seeded at 70% confluency and transfected with siRNA against the p107 (RBL1) transcript or a control sequence. After 48 hours, cells were split into 0 or 350 nM doxorubicin and harvested after 24 hours.
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| Contributor(s) |
Schade AE, Fischer M, DeCaprio JA |
| Citation(s) |
31667499 |
| |
| Submission date |
Aug 14, 2019 |
| Last update date |
Jan 09, 2020 |
| Contact name |
Amy E Schade |
| E-mail(s) |
[email protected]
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| Organization name |
Brigham and Women's Hospital
|
| Department |
Medicine
|
| Lab |
Karen Cichowski
|
| Street address |
77 Avenue Louis Pasteur
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA560251 |
| SRA |
SRP218370 |
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