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Series GSE135771 Query DataSets for GSE135771
Status Public on Mar 13, 2020
Title Evolutionary conserved NSL complex/BRD4 axis controls transcription activation via histone acetylation
Organism Drosophila melanogaster
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Cells rely on a diverse repertoire of genes for maintaining homeostasis, but the transcriptional networks underlying their expression remain poorly understood. The MOF acetyltransferase-containing Non-Specific Lethal (NSL) complex is a broad transcription regulator. It is essential in Drosophila and haploinsufficiency of the human KANSL1 subunit results in the Koolen-de Vries syndrome. Here, we perform a genome-wide RNAi screen and identify the BET protein BRD4 as evolutionary conserved co-factor of the NSL complex. Using Drosophila and mouse embryonic stem cells, we characterise a recruitment hierarchy, where NSL-deposited histone acetylation induces BRD4 recruitment for transcription of constitutively active genes. Transcriptome analyses in Koolen-de Vries patient-derived fibroblasts reveals perturbations with a cellular homeostasis signature that are evoked by the NSL complex/BRD4-axis. We propose that BRD4 represents a conserved bridge between the NSL complex and transcription activation and provide a new perspective in the understanding of their functions in healthy and diseased states.
 
Overall design ChIP-seq profiles of Biotin-tagged dBRD4,endogenous dBRD4, H4K16ac, H3 and Rpb3 from Drosophila melanogaster cells (S2 cells)

Please note that each processed data was generated from both replicates and is linked to the corresponding rep1 sample records.
 
Contributor(s) Gaub A, Akhtar A
Citation(s) 32382029, 37399316
Submission date Aug 13, 2019
Last update date Sep 11, 2023
Contact name Asifa Akhtar
E-mail(s) [email protected]
Organization name Max Planck Institute of Immunobiology and Epigenetics
Department Chromatin Regulation
Lab Akhtar Lab
Street address Stuebeweg 51
City Freiburg
ZIP/Postal code 79108
Country Germany
 
Platforms (2)
GPL19132 Illumina NextSeq 500 (Drosophila melanogaster)
GPL23323 Illumina HiSeq 3000 (Drosophila melanogaster)
Samples (50)
GSM4029602 Dm_S2cell_Biotin_dBRD4-S_ChIPseq_WT_untagged_control_rep1_Input
GSM4029603 Dm_S2cell_Biotin_dBRD4-S_ChIPseq_GST_RNAi_rep1_Input
GSM4029604 Dm_S2cell_Biotin_dBRD4-S_ChIPseq_NSL1_RNAi_rep1_Input
This SubSeries is part of SuperSeries:
GSE135815 Evolutionary conserved NSL complex/BRD4 axis controls transcription activation via histone acetylation
Relations
BioProject PRJNA560036
SRA SRP218224

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE135771_RAW.tar 3.1 Gb (http)(custom) TAR (of BIGWIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
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