|
| Status |
Public on Dec 19, 2020 |
| Title |
Assessing efficiency of single cell population by Drop-seq using cell mixture of epithelial and myeloid cells. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
A lung epithelial cell line was mixed with monocytes/marcophages, and then cells were separated using specific antibodies and/or Drop-seq. PC9 is a non-small cell lung carcinoma (NSCLC) cell line. Myeloid lineage cell line U937 are known to differentiate along monocyte/marcophage lineage by TPA. Using Drop-seq, we were able to clearly distinguish PC9 from U937 cells in the mixture. The separation by Drop-seq was correlated with the separation by cell type-specific antibodies. We were able to identify specific biomarkers, as preferentially expressed genes, for each cell population. The results of our study addresses the quality of cell separation by Drop-seq.
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| |
|
| Overall design |
efficincy of cell separation using single cell RNA-seq
|
| |
|
| Contributor(s) |
Benevolenskaya EV, Aissa AF, Islam AB |
| Citation(s) |
33712615 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 CA211095 |
Role of KDM5A in pRB-mediated differentiation |
BOARD OF TRUSTEES OF THE UNIVERSITY OF ILLINOIS |
Elizaveta V. Benevolenskaya |
|
| |
| Submission date |
Jul 25, 2019 |
| Last update date |
Mar 22, 2021 |
| Contact name |
Elizaveta V Benevolenskaya |
| E-mail(s) |
[email protected]
|
| Phone |
312-413-8947
|
| Organization name |
University of Illinois at Chicago
|
| Department |
Biochemistry and Molecular Genetics
|
| Street address |
900 S. Ashland Ave.
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60607 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
|
| Samples (3) |
|
| This SubSeries is part of SuperSeries: |
| GSE149383 |
Drug resistant changes at the level of single cells |
|
| Relations |
| BioProject |
PRJNA556616 |
| SRA |
SRP216365 |
