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| Status |
Public on Jun 10, 2021 |
| Title |
The effect of aging on the transcriptional profile of female mice exposed to x-ray radiation |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Background: In the event of an improvised nuclear device or radiological dirty bomb detonation large numbers of people will be exposed to radiation that would require a timely and accurate biodosimetry to identify the highly exposed individuals who would require medical treatment from those who have received low or no radiation (the “worried well”). Gene expression signatures in response to radiation have been derived from mice and human peripheral blood and the impact of a number of variables, such as dose, time, genotype, and gender, on gene expression has been analyzed. However, the impact of aging on radiation gene profiling has not been taken into consideration.
Results: Global gene expression was measured in the blood of young (2 mo) and old (24 mo) female C57BL/6J mice 1 day after they were exposed to 4 Gy x-rays or they were sham irradiated (control) using the Agilent Mouse Whole Genome microarrays. Ingenuity Pathway Analysis revealed that “Integrin signaling” was the most overrepresented canonical pathway in both young (p = 1.69E-10) and old (p = 2.93E-10) female mice exposed to x-ray irradiation. In contrast, immune-related processes, such as “Communication between Innate and Adaptive Immune Cells” (p = 2.33E-11), were overrepresented in young but not old irradiated mice. On the other hand, “Molecular Mechanisms of Cancer” processes were significant only in irradiated old mice (p = 5.78E-07). Finally, a number of phagocytosis pathways, involved in apoptotic cell elimination and tissue homeostasis preservation, were enriched in irradiated young, but not old, mice.
Conclusions: We show that age is a variable that has the potential to fundamentally alter the transcriptomic profile of irradiated female mouse blood. A number of biological processes, including phagocytosis, are differentially represented in young and old mice exposed to x-ray radiation. Our results highlight the significance of age as a variable that can have profound biological effects that will affect medical management and treatment decisions in case of a radiological emergency.
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| Overall design |
Radiation induced gene expression in blood from young (2 month) and old (24 months) female mice was measured 24 h after 4 Gy of x-rays. Five independent experiments were performed at each age.
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| Contributor(s) |
Broustas CG |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Jun 27, 2019 |
| Last update date |
Jun 11, 2021 |
| Contact name |
Constantinos Broustas |
| Organization name |
Columbia University
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| Department |
Center for Radiological Research
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| Street address |
630 168th Street
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| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10032 |
| Country |
USA |
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| Platforms (1) |
| GPL11202 |
Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Probe Name version) |
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| Samples (20)
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| GSM3908743 |
Blood, Female, Young-UnIrradiated-rep4 |
| GSM3908744 |
Blood, Female, Young-UnIrradiated-rep5 |
| GSM3908745 |
Blood, Female, Young-Irradiated-rep1 |
| GSM3908746 |
Blood, Female, Young-Irradiated-rep2 |
| GSM3908747 |
Blood, Female, Young-Irradiated-rep3 |
| GSM3908748 |
Blood, Female, Young-Irradiated-rep4 |
| GSM3908749 |
Blood, Female, Young-Irradiated-rep5 |
| GSM3908750 |
Blood, Female, Old-UnIrradiated-rep1 |
| GSM3908751 |
Blood, Female, Old-UnIrradiated-rep2 |
| GSM3908752 |
Blood, Female, Old-UnIrradiated-rep3 |
| GSM3908753 |
Blood, Female, Old-UnIrradiated-rep4 |
| GSM3908754 |
Blood, Female, Old-UnIrradiated-rep5 |
| GSM3908755 |
Blood, Female, Old-Irradiated-rep1 |
| GSM3908756 |
Blood, Female, Old-Irradiated-rep2 |
| GSM3908757 |
Blood, Female, Old-Irradiated-rep3 |
| GSM3908758 |
Blood, Female, Old-Irradiated-rep4 |
| GSM3908759 |
Blood, Female, Old-Irradiated-rep5 |
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| Relations |
| BioProject |
PRJNA551475 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE133451_RAW.tar |
177.5 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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