|
| Status |
Public on Jun 22, 2019 |
| Title |
Ribosome profiling of Cryptococcus neoformans H99 |
| Organisms |
Cryptococcus neoformans; Cryptococcus neoformans var. neoformans |
| Experiment type |
Expression profiling by high throughput sequencing
Other
|
| Summary |
We measured protein translation (by ribosome profiling) and RNA levels (by polyA-enriched RNA-seq) in Cryptococcus neoformans strain H99 and Cryptococcus neoformans strain JEC21. This is the first transcriptome-wide map of translation in this species complex.
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| |
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| Overall design |
Matched ribosome profiling and RNA-seq from 6 biological samples: 2x wild-type H99 and 2 deletion mutants (Ago1, Gwo1), and 1x wild-type JEC21 and 1x deletion mutant (Ago1).
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| |
|
| Contributor(s) |
Sales-Lee J, Madhani H, Wallace E |
| Citation(s) |
32020195 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 AI120464 |
Epigenetic control of virulence in a fungal meningitis pathogen |
University of California San Francisco |
Hiten D Madhani |
| R01 GM071801 |
Mechanisms of Gene Silencing |
University of California San Francisco |
Hiten D Madhani |
|
| |
| Submission date |
Jun 21, 2019 |
| Last update date |
Mar 16, 2020 |
| Contact name |
Edward William Joseph Wallace |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Edinburgh
|
| Department |
Institute for Cell Biology, School of Biological Sciences
|
| Street address |
Waddington Building, Max Born Crescent
|
| City |
Edinburgh |
| ZIP/Postal code |
EH9 3BF |
| Country |
United Kingdom |
| |
|
| Platforms (2) |
| GPL19081 |
Illumina HiSeq 2500 (Cryptococcus neoformans) |
| GPL26812 |
Illumina HiSeq 2500 (Cryptococcus neoformans var. neoformans) |
|
| Samples (12)
|
|
| Relations |
| BioProject |
PRJNA550038 |
| SRA |
SRP202195 |
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