|
| Status |
Public on Oct 22, 2008 |
| Title |
LSD1 knock down in SY5Y Cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
To analyze the functional relevance of LSD1 in neuroblastic tumors, SH-SY5Y cells were transiently transfected with siRNA directed against LSD1 or with a scrambled control siRNA. Microarray analysis revealed changes in expression that were consistent with these observations 72 hours after LSD1 knock-down. At this time, 28 genes were significantly induced at least 1.5-fold and 29 genes were significantly repressed at least 1.5-fold. Among the 28 induced genes, 4 are markers of cytoskeletal remodelling (TNS1, TPM1, DNM2, DNAL4), indicating differentiation, and 3 (TPM1, DNM2 and SHANK2) are functionally linked to neurite dynamics and synaptic trafficking. TaqMan quantitative RT-PCR confirmed the expression changes detected via microarray analysis for LSD1, DNAL4, DNM2, TNS1 and TPM1
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|
| Overall design |
SH-SY5Y cells were transfected with siRNA directed against LSD1 or control siRNA. 2 independent transfection experiments were performed each. Reverse transcription, labelling of total RNA and subsequent hybridization to Affymetrix U133v2 chips were performed according to the manufacturer´s protocols
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| |
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| Contributor(s) |
Schulte JH, Krifel J, Klein-Hitpaß L |
| Citation(s) |
19223552 |
| |
| Submission date |
Oct 19, 2008 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Johannes Hubertus Schulte |
| E-mail(s) |
[email protected]
|
| Phone |
004920172385185
|
| Fax |
00492017235750
|
| URL |
http://www.uk-essen.de
|
| Organization name |
University Children's Hospital Essen
|
| Department |
Peadiatric Hematology and Oncology
|
| Lab |
Peadiatric Hematology and Oncology
|
| Street address |
Hufelandstr.55
|
| City |
Essen |
| State/province |
NRW |
| ZIP/Postal code |
45130 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
|
| Samples (4)
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| Relations |
| BioProject |
PRJNA109589 |
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